Potential role of root-associated bacterial communities in adjustments of desert plant physiology to osmotic stress

Abstract

Plants possess the ability to adapt to osmotic stress by adjusting their physiology and morphology and by cooperating with their root-associated (rhizosphere and endosphere) microbial communities. However, the coordination of host self-regulation with root-associated microorganisms at the community level, especially for desert plants, remains unclear. This study investigated the morphophysiological responses of seedlings from the desert plant Alhagi sparsifolia Shap to osmotic stress, as well as the relationships between these adaptations and their root-associated bacterial communities. The results indicated that osmotic stress contributed to a reduction in height and increased levels of reactive oxygen species (ROS) and malondialdehyde (MDA). In response, A. sparsifolia exhibited a series of morphophysiological adjustments, including increased ratio of root to shoot biomass (R/S) and the number of root tip, enhanced vitality, high levels of peroxidase (POD), ascorbate peroxidase (APX), and glutathione (GSH), as well as osmolytes (proline, soluble protein, and soluble sugar) and modification in phytohormones (abscisic acid (ABA) and jasmonic acid (JA)). Additionally, osmotic stress resulted in alterations in the compositions and co-occurrence patterns of root-associated bacterial communities, but not α-diversity (Chao1). Specifically, the rhizosphere Actinobacteria phylum was significantly increased by osmotic stress. These shifts in root-associated bacterial communities were significantly correlated with the host's adaptation to osmotic stress. Overall, the findings revealed that osmotic stress, in addition to its impacts on plant physiology, resulted in a restructuring of root-associated microbial communities and suggested that the concomitant adjustment in plant microbiota may potentially contribute to the survival of desert plants under extreme environmental stress.