Potential risks of nicotine on the germination, growth, and nutritional properties of broad bean

Abstract

This study evaluated the allelopathy, uptake and accumulation, and potential agricultural and food safety risks of nicotine in broad bean (Vicia faba L.) during seed germination and seedling growth. Nicotine stress has an allelopathic inhibitory effect on seeds and a hormesis effect on germinated seeds and seedlings, which has an enhancement effect (<50 mg kg−1) and an inhibition effect (>100 mg kg−1) on the germinated seeds and an enhancement effect (<100 mg kg−1) and an inhibition effect (>200 mg kg−1) on the seedlings. Exogenous nicotine can be absorbed by broad bean roots from nicotine-contaminated soil and accumulated in the main organs of the seedlings, especially the leaves, which exceeded the maximum residue level (0.03 mg kg−1 DW) at 50 mg kg−1. Moreover, nicotine resulted in a bitter taste in the edible broad bean leaves, disrupting the balance of basic nutritional properties, decreasing sucrose, and increasing bitter substances such as choline and procyanidin. These results demonstrated that residual nicotine in the soil not only poses potential risks to sustainable agricultural development but also a food safety risk for consumers. The present study provides insight into the potential risks of nicotine in agroecosystems.