Abstract
To investigate the potential harmful effects of potassium dichromate and magnesium sulphate causing oxidative stress and reproductive toxicity in adult male mice model. The experimental work was conducted on sixty male mice (Mus musculus) divided into three groups. Mice in group B and C received potassium dichromate and magnesium sulphate of 5.0 and 500 mg/Kg body weight/ml respectively, for sixty days. The blood sample was analyzed to assess oxidative stress and cellular damage. RESULTS showed high malondialdehyde (MDA) and low levels of antioxidant enzymes [catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx)] in both potassium dichromate and magnesium sulphate administrated groups as compared to control group. Reduced number of sperm count and excessive destruction of testicular follicles, including destruction of spermatids, leydig cells and sertoli cells, were also seen in both groups. We concluded from present study that potassium dichromate and magnesium sulphate causes oxidative stress by generation of reactive oxygen species (ROS) and causing DNA damage in testicular cells leading to adverse reproductive abnormalities.
Highlights
Worldwide distribution and extensive use of chemical agents, is associated with concern of the highest priority for environmental and industrial exposure which may have imposing effects on Correspondence: *1st Revision Received: 2nd Revision Received: December 19, 2013 April 7, 2014 April 14, 2014 April 18, 2014 male reproductive function
Group A served as control, while in group B mice were treated with K2Cr2O7 and in group C mice were administered with MgSO4
Samples collection and sample analysis: 2 ml blood was taken from each mouse at 1st, 30th and 60th day of the experiment and serum of the sample was separated by centrifugation at 3000 rpm
Summary
Lipid peroxidation is one of the major outcomes of free radical-mediated to cellular injury or beneficial biological effects. K2Cr2O7 and MgSO4 both induce oxidative stress due to which high lipid peroxidation occurs and in result MDA level is increased and antioxidant enzymes SOD and CAT activity is decreased.[6,7] The evidence indicates that the careless use of toxic heavy metals in the past twenty years have shown very alarming trend in male reproductive health.[8] Testicular tissues are major target organ for metals that induce oxidative damage because of its high contents of polyunsaturated membrane lipids. The aim of this research work was to check the adverse effects of K2Cr2O7 and MgSO4 on the testes of adult male mice
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