Abstract
Diazinon (DZN), is a commonly used organophosphorous (OP) pesticide to control a variety of insects in agriculture and in the environment. Vitamin E is a primary antioxidant that plays an important role in protecting cells against toxicity by inactivating free radicals generated following pesticides exposure. Therefore, the present study was undertaken to investi- gate the possible protective effect of vitamin E against DZN- induced adverse effects on haematological and biochemical indices and on genotoxicity using comet assay and micronucleus test for measuring the DNA damage. The tissue DZN residues in liver, kidney and muscle of the rats were determined. Vitamin E (200 mg/kg, twice a week), diazinon (10mg/kg/ day, once a day), and vitamin E (200mg/kg, twice a week + diazinon (10mg/kg/day, once a day) combination were given to rats (n= 10) orally via gavage for 4 weeks. The results revealed that DZN administration significantly decreased Hb concentration, RBCs count and PCV values. Meanwhile, a significant increased in WBCs, ALT, AST and total cholesterol was detected. However, vitamin E supplementation together with DZN improves these alterations. DZN residues level is highest in the kidney than that in liver and muscle tissues. Administration of vitamin E together with DZN reduces the residual values in the examined tissues. A significant increase in tail length of comets from blood cells as well in the fre- quency of micronucleated cells (MNCs) following DZN administration was achieved. Co-administrated vitamin E along with DZN resulted in decrease in tail length of comets and the percentage of MNCs compared to DZN alone treated rats. The increase in frequency of MNCs and tail length of comets confirm the genotoxicity of DZN. Vitamin E, on the other hand, was observed to repair the genotoxicity and improves the haematological and biochemical changes induced by DZN. It can be concluded that vitamin E has protective effect against DZN adverse effects and supplementation of vitamin E might be beneficial to DZN exposed populations.
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