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Abstract
Ultraviolet B (UVB) irradiation causes adverse effects on the skin. Corn silk contains flavonoids and other bioactive compounds and antioxidants, which may prevent skin photoaging through antioxidant and anti-inflammatory effects. We aimed to investigate the potential photoprotective effects of dietary corn silk on UVB-induced skin damage in mice and the mechanisms behind these effects on human skin cells. Oral administration of corn silk water extract (CS) (2 or 4 g/kg/day) for 19 weeks decreased epidermal thickness, wrinkle formation, and positive staining for PCNA, Ki67, and 8-OHdG, and increased collagen staining in UVB-irradiated SKH-1 hairless mice compared with controls. The pro-inflammatory NF-κB target genes (IL-1β, iNOS, and COX-2) and MMP-9 expressions were lower in the CS groups, and TGF-β/Smad signaling increased. Low skin lipid peroxidation and blood DNA oxidation levels and high blood glutathione were detected. Antioxidant transcription factor Nrf2-related catalase and SOD1 proteins and glutaredoxin mRNA levels increased. The results of CS extract treatment and UVB irradiation in HaCaT cells showed the same results in Nrf2 and NF-κB target genes. An LC-MS/MS analysis showed that the CS extract contained potential antioxidants, which might have contributed to its anti-photoaging effects in tissues and cells. CS extract may reduce UVB-induced skin damage through antioxidant and anti-inflammatory mechanisms.
Highlights
Skin, the largest organ in the human body, acts as a barrier against external pollutants [1].Skin tissue is constantly exposed to solar ultraviolet (UV) radiation [2]
We aimed to demonstrate the effect of corn silk water extract (CS) extract on preventing UVB-irradiated group (UVB)-induced skin damage in mice and further confirm the molecular mechanisms underlying this effect in human skin cells
CS extracts at concentrations of 1.25, 2.5, 5, 10 mg/mL and DPPH or ABTS solution were mixed at a ratio of 1:4 and 1:99
Summary
The largest organ in the human body, acts as a barrier against external pollutants [1].Skin tissue is constantly exposed to solar ultraviolet (UV) radiation [2]. When the antioxidant defense system is damaged due to ultraviolet B (UVB) exposure of the skin, excessive reactive oxygen species (ROS) are generated at the irradiated sites [3], which results in various changes, including the oxidation of cellular components, DNA mutation, inflammation, and the activation of specific enzymes that degrade the extracellular matrix [4]. These changes can lead to unrestrained cell proliferation [5]. There are related histological characteristics such as disorganization of collagen in the dermis, which lies below the epidermis, fragmentation, and dispersion [7]
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