Abstract
Pandemic SARS-CoV-2 (COVID-19) is a severe problem in the world today. The SARS-CoV-2 virus contains protease and glycoprotein spike, which was used infection and development. The RBD (Receptor Binding Domain) of the glycoprotein Spike (RBD-S) can bind to the ACE2 receptor (Angiotensin Converting Enzyme-2) on the Domain protease (PD) (PD-ACE2) of the host cell causing virus infection. This study aimed to evaluate the potential of bioactive peptides from tilapia viscera hydrolysate as an antiviral peptide to attempt a simulated docking with four protein target virus COVID 19. The research was conducted by molecular docking using the PyRx software. Selected protein targets were SARS-CoV-2 protease (GDP ID: 6LU7), SPIKE COVID 19 (PDB ID: 6LVN), ACE2 (GDP ID: 6VW1), and deubiquitinase inhibitors block the SARS virus replication (PDB ID: 3E9S). The formed binding affinity was represented as a docking score. The results showed that both the Asp-Trp and Val-Tyr peptides were potential as SARS-CoV-2 antiviral, with an affinity strength equal to chloroquine and favipiravir. The Asp-Trp and Val-Tyr peptides could bind to all four receptor proteins target on the active side. Therefore, it potentially inhibits the virus sticking to proteins target that results in inhibition of virus replication. Generally, the Asp-Trp and Val-Tyr peptides of tilapia viscera hydrolysate are potential as an alternative antiviral peptide to medicate the infections and replication of COVID-19.
Highlights
Aquaculture waste such as viscera contain highly protein and unsaturated fatty acids [1]. fish viscera can be utilized as a raw material for hydrolysate proteins [2]
This study aimed to evaluate the potential of bioactive peptides from tilapia viscera hydrolysate as an antiviral peptide to attempt a simulated docking with four protein target virus COVID 19
The results showed that both the Asp-Trp and Val-Tyr peptides were potential as SARS-CoV-2 antiviral, with an affinity strength equal to chloroquine and favipiravir
Summary
Aquaculture waste such as viscera contain highly protein and unsaturated fatty acids [1]. fish viscera can be utilized as a raw material for hydrolysate proteins [2]. Fish viscera can be utilized as a raw material for hydrolysate proteins [2]. Aquaculture waste such as viscera contain highly protein and unsaturated fatty acids [1]. It can minimize environmental and health issue and reduce the economic impact [3]. One of the efforts to utilize fish waste is using hydrolysis technology. Hydrolysis technology breaks down complex bonds into simple bonds in the form of bioactive peptides using enzymes, acids, and bases [4]. Bioactive peptides are specific protein that possess a health benefit. Proteins in the whole form have weak bioactivity, whereas hydrolyzed proteins will increase biological activity because it has been separated from the complex bonds [5]
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