Potential of synchronous fluorescence spectroscopy coupled with chemometrics to determine the heterocyclic aromatic amines in grilled meat

Abstract

In this study, the potential of synchronous front-face fluorescence spectroscopy (SFS) coupled with chemometrics was investigated for the determination of heterocyclic aromatic amines (HAA) in cooked meat samples. Bovine meat samples (1–2 mm thick, 5 cm diameter) taken from Longissimus thoracis muscle were cooked at an average temperature of 237 °C for 5, 7 and 10 min. Four HAA (4, 8-DiMeIQx, MeIQx, IQx and PhIP) were determined on the cooked meat samples using classical LC-APCI-MS/MS method. In parallel, SFS spectra were recorded using a spectrofluorimeter on the same cooked meat samples in an excitation wavelength range of 250–550 nm using offsets of 20, 30, 40, …, 160 nm between excitation and emission monochromators. The three-dimensional synchronous fluorescence data set was analyzed using PARAFAC (parallel factor) analysis and N-PLS (n-way partial least square) regression method. PARAFAC analysis allowed capturing the fluorescence changes occurring in meat during cooking: the best model was obtained with 2 components (core consistency of 100% and explained variance of 99.2%). Whereas the loading profile of component 1 showed a maximum excitation at about 495 nm and an optimal offset of 60 nm, the loading profile of component 2 was characterized by a maximum excitation at 367 nm and an optimal offset of 90 nm. The results obtained using N-PLS regression showed good correlation between the spectral and analytical data: average recovery of 104% for 4, 8-DiMelQx, 102% for both MelQx and IQx, and 103% for PhIP were obtained. In conclusion this study indicates that SFS along with chemometrics has the potential to be used as a rapid and non-destructive technique for the determination of HAA contents in meat.