Potential of Smoke-Water and One of Its Active Compounds (karrikinolide, KAR1) on the Phytochemical and Antioxidant Activity of Eucomis autumnalis.

Adeyemi Oladapo Aremu,Johannes Van Staden,Karel Doležal,Jiri Gruz,Nqobile Andile Masondo

doi:10.3390/antiox8120611

Abstract

Eucomis autumnalis (Mill.) Chitt. subspecies autumnalis is a popular African plant that is susceptible to population decline because the bulbs are widely utilized for diverse medicinal purposes. As a result, approaches to ensure the sustainability of the plants are essential. In the current study, the influence of smoke-water (SW) and karrikinolide (KAR1 isolated from SW extract) on the phytochemicals and antioxidant activity of in vitro and greenhouse-acclimatized Eucomis autumnalis subspecies autumnalis were evaluated. Leaf explants were cultured on Murashige and Skoog (MS) media supplemented with SW (1:500, 1:1000 and 1:1500 v/v dilutions) or KAR1 (10−7, 10−8 and 10−9 M) and grown for ten weeks. In vitro regenerants were subsequently acclimatized in the greenhouse for four months. Bioactive phytochemicals in different treatments were analyzed using ultra-high performance liquid chromatography (UHPLC-MS/MS), while antioxidant potential was evaluated using two chemical tests namely: DPPH and the β-carotene model. Smoke-water and KAR1 generally influenced the quantity and types of phytochemicals in in vitro regenerants and acclimatized plants. In addition to eucomic acid, 15 phenolic acids and flavonoids were quantified; however, some were specific to either the in vitro regenerants or greenhouse-acclimatized plants. The majority of the phenolic acids and flavonoids were generally higher in in vitro regenerants than in acclimatized plants. Evidence from the chemical tests indicated an increase in antioxidant activity of SW and KAR1-treated regenerants and acclimatized plants. Overall, these findings unravel the value of SW and KAR1 as potential elicitors for bioactive phytochemicals with therapeutic activity in plants facilitated via in vitro culture systems. In addition, it affords an efficient means to ensure the sustainability of the investigated plant. Nevertheless, further studies focusing on the use of other types of antioxidant test systems (including in vivo model) and the carry-over effect of the application of SW and KAR1 for a longer duration will be pertinent. In addition, the safety of the resultant plant extracts and their pharmacological efficacy in clinical relevance systems is required.

Highlights

Indigenous people have recognized the role of fire and smoke on plant cultivation and productivity [1,2]
Eucomic Acid in In Vitro and Greenhouse-Acclimatized Plants. Both the in vitro and greenhouse-acclimatized Eucomis autumnalis subspecies autumnalis accumulated varying levels of eucomic acid, which were generally higher in the acclimatized plants compared to the in vitro regenerants (Figure 1)
The importance of SW and KAR1 as potential elicitors for bioactive phytochemicals was demonstrated in Eucomis autumnalis subspecies autumnalis

Summary

Introduction

Indigenous people have recognized the role of fire and smoke on plant cultivation and productivity [1,2]. Several types of KAR, generally referred to as karrikins, have been identified, and are recognized as a new family of plant growth regulators (PGRs) [8] Both SW and KAR1 are known to interact with other PGRs [8] and often exhibit cytokinin and auxin-like activities, as demonstrated in the mungbean bioassay [9]. Phytochemicals have received increasing attention due to their antioxidant properties and ability to counteract oxidative stress associated with various diseases [16] As a result, these bioactive phytochemicals have been widely-explored for their therapeutic and pharmacological (e.g., antioxidant, antimicrobial, anti-inflammatory, and anti-diabetics) value, as evidenced in several African medicinal plants [17]

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Discussion

Conclusion