Abstract
The development of Jembrana disease vaccine is importance to prevent the loss of Bali cattle industry in Indonesia. This study aims to prepare a Jembrana DNA vaccine. The data Tat protein sequences gained from NCBI and the consensus process has been finished by the MultAlign program, and then Cloning of the pcDNA3.1-tat has been successfully performed on E. coli DH5α and confirmed by PCR, restriction analysis and sequencing. The propagated plasmids were prepared as DNA-chitosan complex and physiochemical characterized using Particle Size Analyzer. Complex with a 1:2 (wt/wt) ratio of DNA and chitosan have a mean diameter of 268.5 nm and zeta potential +25.1 mV and the value of Cytotoxicity Assay 80-90% as compared to the untreated cells that used as negative control, so it can be concluded that nanoparticles chitosan has good potential as a carrier agent for pcDNA3.1-tat.
Highlights
To meet the needs of the Indonesian people for the source of meat protein, an effort is made to increase the beef cattle population
A DNA vaccine against Jembrana Disease Virus was constructed based on the consensus sequence from amino acid sequences of Tat1 protein
This study will use the pcDNA3.1(+) vector to encode a synthetic gene constructed from a consensus sequence of one gene from Jembrana Disease Virus (JDV)
Summary
To meet the needs of the Indonesian people for the source of meat protein, an effort is made to increase the beef cattle population. The effort to increase the population of Bali cattle is hampered by diseases caused by viruses, one of which is Jembrana disease, the only infectious disease attacking Bali Cattle. The vaccine has several drawbacks, such as limited supply of materials and complicated isolation techniques that take time. This encourages experts to develop vaccines through recombinant DNA techniques. The vaccine is made by entering DNA or genes that encode immunogenic proteins into eukaryotic expression vectors
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