Abstract
The purpose of this study to determine the ability of Chlorella vulgaris in absorbing Pb (lead) and the effect of the variation of Pb metal concentration on the growth of Chlorella vulgaris.This study using an experimental study with complete random design with 4 treatments, namely control (without the addition of metal), Pb1 (addition of metal 1 mg / l), Pb3 (3 mg / l) and Pb5 (5 mg / l), respectively 3 replications. Exposure Pb ion in Chlorella vulgaris for 7 days. Analysis of the metal content of Pb concentration performed on culture media after exposure it at 3 hours after dispersion Chlorella vulgaris and on day 7 of culture using the AAS method. Do also counting the growth of cells each day. The results of the analysis of the average metal content of Pb in the culture medium at the end of the study was the control (0.1980), Pb1 (0.1453), Pb3 (0.4144) and Pb5 (0.5305). While the average growth of Chlorella vulgaris at the end of the study were control (630.1116 x 104), Pb1 (829.0012 x 104), Pb3 (1069.9446 x 104) and Pb 5 (808.94450 x 104). The results of the analysis of the content of Pb in the F test shown that the difference in concentration of water Pb given real influence on the ability of Chlorella vulgaris in absorbing Pb and growth. The conclusion of this study was Chlorella vulgaris has the ability to absorb metals in the waters, and the provision of various concentrations of Pb can affect the growth of Chlorella vulgaris.
Highlights
Control of the environment from pollution by industrial waste is one of the issues that need to be addressed, because it is potentially causing changes in water quality due to the influx of waste from these activities, industrial waste containing heavy metals, which will lead to increasing pollutants carried by streams towards the estuary and will accumulate in the ocean
One of the sewage treatment process to reduce pollution by heavy metals problem is to bioremediation, which is the process of converting waste contamination in an attempt to repair the quality of the environment by utilizing living things
To get cell density of ± 1 x 106 cells / ml, the dilution calculation using the formula as follows: V1 x V2 x N1 = N2 Information: V1 = Volume of Chlorella vulgaris necessary for culture N1 = density cell culture seedlings on stock V2 = volume of seawater used as a culture medium N2 = density desired cells for culture (6)
Summary
Control of the environment from pollution by industrial waste is one of the issues that need to be addressed, because it is potentially causing changes in water quality due to the influx of waste from these activities, industrial waste containing heavy metals, which will lead to increasing pollutants carried by streams towards the estuary and will accumulate in the ocean. These problems can affect the marine life that is therein to impact the lives of human dependence on marine environment is very large. Where the use of Chlorella vulgaris has not been maximized, only limited as a dietary supplement and ingredient manufacture of cosmetics
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