Abstract

Bioprospecting study of indigenous microbial community is still being approached especially for some microbial strains in bioremediation aspect. The objective of this study was to obtain a collection of indigenous bacterial and fungal isolates capable of growing on hydrocarbon medium. Soil samples were collected from Terjun Landfill located in Medan City, North Sumatra. Mineral salt agar medium supplemented with 10% (v/v) of hydrocarbon sources (diesel oil, pesticide, dye wastewater) was used to screen the microbial capacity in utilizing the hydrocarbon compounds as sole carbon source. Each isolate was categorized based on their growth performance or colony diameter on agar medium namely none (-), slow (+), moderate (++), and rapid (+++). The results obtained a total of 27 bacterial isolates and 6 fungal isolates. Majority of microbial isolates showed a better growth on pesticide medium than in diesel oil and dye wastewater which indicated their capability in converting the pesticide as nutrient. It is worthwhile to investigate the further application of our indigenous strains in contaminated areas in order to ensure the environmental sustainability.

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