Potential of Lactobacillus plantarum IBB3036 and Lactobacillus salivarius IBB3154 to persistence in chicken after in ovo delivery.

Tamara Aleksandrzak‐Piekarczyk,Jacek K Bardowski,Roman K Górecki,Krzysztof A Gulewicz,Weronika Puzia,Jarosław Cieśla,Joanna Żylińska

doi:10.1002/mbo3.620

Abstract

The aim of this study was to characterize and compare selected Lactobacillus strains originating from different environments (cow milk and hen feces) with respect to their applicative potential to colonize gastrointestinal track of chickens before hatching from an egg. In vitro phenotypic characterization of lactobacilli strains included the investigation of the important prerequisites for persistence in gastrointestinal tract, such as a capability to survive in the presence of bile salts and at low pH, enzymatic and sugar metabolic profiles, adhesion abilities, and resistance to osmolytes, temperature, and antibiotics. Regarding the resistance of lactobacilli to most of the various stress factors tested, the milk isolate Lactobacillus plantarum IBB3036 showed better abilities than the chicken feces isolate Lactobacillus salivarius IBB3154. However, regarding the acidification tolerance and adherence ability, L. salivarius IBB3154 revealed better characteristics. Use of these two selected lactobacilli isolates together with proper prebiotics resulted in the preparation of two S1 and S2 bioformulations, which were injected in ovo into hen Cobb500 FF fertilized eggs. Furthermore, in vivo tests assessing the persistence of L. plantarum IBB3036 and L. salivarius IBB3154 in the chicken gastrointestinal tract was monitored by PCR‐based classical and quantitative techniques and revealed the presence of both strains in fecal samples collected 3 days after hatching. Subsequently, the number of L. salivarius IBB3154 increased significantly in the chicken intestine, whereas the presence of L. plantarum IBB3036 was gradually decreased.

Highlights

Lactic acid bacteria (LAB) are important autochthonous residents in the chicken gastrointestinal tract (GIT) (Musikasang, Tani, H-kittikun, & Maneerat, 2009)
The study of acid tolerance showed that cells of both L. plantarum IBB3036 and L. salivarius IBB3154 strains were vital after an incubation period of 3 hr, at pH 3.5 and pH 2.5
Some authors have promoted the importance of an intestinal origin as a selective criterion for the search for desirable strains, but the expert FAO-WHO (2006) panel suggested that probiotic activity is more relevant than the source of the bacteria

Summary

| INTRODUCTION

Lactic acid bacteria (LAB) are important autochthonous residents in the chicken gastrointestinal tract (GIT) (Musikasang, Tani, H-kittikun, & Maneerat, 2009). Since the role of LAB in the feed conversion efficacy and health of the birds was recognized, Lactobacillus strains have been extensively studied and used as synbiotics, which refer to a mixture of probiotic and prebiotic compounds (Ehrmann, Kurzak, Bauer, & Vogel, 2002; Patterson & Burkholder, 2003). Their synergistic effects can potentiate the implantation and persistence of desirable bacteria in animal GIT. The persistence of the two selected Lactobacillus strains in the gut was monitored, using a PCR-based quantitative method showing the presence of both strains in 3-day fecal samples and the considerable species-dependent variability over that time

| MATERIALS AND METHODS

Findings

| DISCUSSION