Abstract
The research on environmental biotechnology has been done in aims to study the ability of mixed culture indigenous bacteria to remove cyanide (KCN-) concentration which is determined by environment conditions of temperature and contact time. The research has been done using the artificial pollutants that contains 500 ppm of KCN- which is exposed to the SMSS (Stone Mineral Salt solution) as a growth media for mixed culture bacteria TBS and CTS. The effect of different environment conditions including temperature and contact time were studied on cyanide removal. Gas Chromatography - Mass Spectrophotometer (GC - MS) method is used to analysed the concentrations of KCN-. Initial condition of research operation is determined to be pH 7 and contact time of 48 hours. The removal of 500 ppm KCN- increases to 71% at incubation temperature 35°C and contact time of 36 hours. This research showed that indigenous bacteria TB1 and CS2 can remove 500 ppm KCN- on all temperature variations between (°C) 25 to 45 and the highest removal occur when contact time is 36 hours.
Highlights
Cyanide contamination can occur in soil, river and ground flow
This study focuses on the effect of incubation temperature and contact time to obtain the highest cyanide removal
This study focuses on the effect of temperature and contact time on cyanide removal
Summary
Cyanide contamination can occur in soil, river and ground flow. Cyanide is one of the chemicals used in gold mining and jewelry industry activities. The area around the gold mining industry is one area that is often exposed to heavy metal contamination and cyanide [1]. Cyanide contamination is common in areas around gold mining industry. The presence of cyanide in water and soil can harm the environment and human health [2]. Cyanide can affect negatively to human and animal health, to the point of causing death at a certain concentration. Required a processing to treat the contamination of the cyanide compound
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