Abstract
Downy mildew (Plasmopara viticola) and powdery mildew (Erysiphe necator) are the most common and economically significant fungal diseases in vineyards. The task of this work is to study the genotypes of new promising hybrid forms of table grapes for the presence of resistance genes to downy mildew (Rpv10 and Rpv3) and powdery mildew (Ren9) using DNA-markers. The study was carried out on table grape hybrids under the working names Agat dubovskiy, Akelo, Arabella, Artek, Dubovskiy rozovyi, Gamlet, Ispolin, Kishmish dubovskiy, Kurazh, Pestryi, Valensiya and registered variety Liviya. The studied genes were analyzed using markers UDV305 and UDV737 (Rpv3), GF09-46 (Rpv10), CenGen6 (Ren9). The following cultivars were used as reference genotypes: Saperavi severnyi (carries Rpv10 gene) and Regent (Rpv3 and Ren9). It was established that Rpv3 gene is carried by hybrids Kishmish dubovskiy, Agat dubovskiy, Kurazh, Valensiya, Akelo, Gamlet, Dubovskiy rozovyi, Pestryi. Ren9 gene was found in Artek, Agat dubovskiy, Kurazh, Ispolin, Valensiya, Arabella, Gamlet, Dubovskiy rozovyi, Pestryi. The Rpv10 gene was not detected in any of the analyzed grapevine samples. genotypes Agat dubovskiy, Kurazh, Gamlet, Dubovskiy rozovyi, Pestryi, Valensiya carry Rpv3 and Ren9 genes simultaneously. These grapevines have an elegant bunch and large berries that are attractive to consumers.
Highlights
The development of DNA-marking methods has fundamentally changed the approaches to the classification and certification of varieties, the assessment of genetic diversity, mapping and identification of genes, genetic monitoring in genetics and breeding of cultivated plants
Table grape genotypes Agat dubovskiy, Akelo, Arabella, Artek, Dubovskiy rozovyi, Gamlet, Ispolin, Kishmish dubovskiy, Kurazh, Pestryi, Valensiya, Liviya were studied by DNAmarkers for the presence of two downy mildew resistance genes (Rpv10 and Rpv3) and one powdery mildew resistance gene (Ren 9)
For the detection of Rpv10 gene, the GF09-46 marker was used [15], target polymerase chain reaction (PCR) fragment indicating the presence of resistance allele did not found in any analyzed genotype (Table)
Summary
The development of DNA-marking methods has fundamentally changed the approaches to the classification and certification of varieties, the assessment of genetic diversity, mapping and identification of genes, genetic monitoring in genetics and breeding of cultivated plants. DNA-markers identification of pathogen resistance genes in new breeding forms and grape varieties makes it possible to determine their adaptive potential to biotic stress factors. The determination of resistance genes in grapevines to these pathogens is carried out mainly in all world scientific centers [1,2,3,4,5,6,7,8,9]. Identification of genes for resistance to downy mildew and powdery mildew in domestic material with the aim of creating stable ampelocenoses, preserving the harvest and extending the productive life of grape plantations is an urgent task
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