Potential of eight mutations for marker-assisted breeding in Chinese Lulai black pigs

Abstract

Molecular marker-assisted selection (MAS) provides an efficient tool for pig breeding. In this study, according to the literature, we selected eight effective or causal mutations from eight functional genes, including five causal mutations in PHKG1 ( rs330928088), MUC13 ( rs319699771), IGF2 ( g.3072 G> A), VRTN ( g.20311_20312ins291), and MYH3 ( XM_013981330.2:g.-1805_-1810del) genes, and three effective mutations in LIPE ( rs328830166), LEPR ( rs45435518), and MC4R ( rs81219178) genes, to investigate their potential breeding effect in 418 Lulai pigs. The linear model was used to analyse the association between mutations and intramuscular fat (IMF) content, average backfat thickness, and muscle moisture %. The results revealed that amongst the three effective mutations, only the mutation in the LEPR gene, which affects IMF deposition, was significantly associated with IMF content. However, the other molecular markers were not significantly associated with the affected traits reported in previous studies, and these mutations are ineffective for MAS in the Lulai black pig population. Therefore, causal mutations in PHKG1, IGF2, and VRTN genes, and an effective mutation in LEPR gene could be used as effective breeding makers for MAS in Lulai pigs. These results can provide helpful information for further breeding in Lulai black pigs.