Abstract
Bacillus thuringiensis ser. israelensis (Bti) has been widely used as microbial larvicide for the control of many species of mosquitoes and blackflies. The larvicidal activity of Bti resides in Cry and Cyt δ-endotoxins present in the parasporal crystal of this pathogen. The insecticidal activity of the crystal is higher than the activities of the individual toxins, which is likely due to synergistic interactions among the crystal component proteins, particularly those involving Cyt1Aa. In the present study, Cry10Aa and Cyt2Ba were cloned from the commercial larvicide VectoBac-12AS® and expressed in the acrystalliferous Bt strain BMB171 under the cyt1Aa strong promoter of the pSTAB vector. The LC50 values for Aedes aegypti second instar larvae estimated at 24 hpi for these two recombinant proteins (Cry10Aa and Cyt2Ba) were 299.62 and 279.37 ng/mL, respectively. Remarkable synergistic mosquitocidal activity was observed between Cry10Aa and Cyt2Ba (synergistic potentiation of 68.6-fold) when spore + crystal preparations, comprising a mixture of both recombinant strains in equal relative concentrations, were ingested by A. aegypti larvae. This synergistic activity is among the most powerful described so far with Bt toxins and is comparable to that reported for Cyt1A when interacting with Cry4Aa, Cry4Ba or Cry11Aa. Synergistic mosquitocidal activity was also observed between the recombinant proteins Cyt2Ba and Cry4Aa, but in this case, the synergistic potentiation was 4.6-fold. In conclusion, although Cry10Aa and Cyt2Ba are rarely detectable or appear as minor components in the crystals of Bti strains, they represent toxicity factors with a high potential for the control of mosquito populations.
Highlights
Bacillus thuringiensis ser. israelensis (Bti) was the first Bt serotype found to be toxic for dipteran species [1]
Insecticidal Cry and Cyt Genes Identified in Bti Strain from VectoBac-12AS®
A bioinformatic analysis of the genome of the Bti strain isolated from the commercial product
Summary
Bacillus thuringiensis ser. israelensis (Bti) was the first Bt serotype found to be toxic for dipteran species [1]. The absence of resistance is likely due to the different modes of action and the synergistic effects of the multiple crystal proteins present in Bti-based products [9,10,11]. Cry10Aa and Cyt2Ba have been described in some Bti strains, these are expressed and accumulate in the crystal in much smaller quantities that the four main components [13,14]. All six of these proteins are encoded in the fully sequenced Bti plasmid pBtoxis [15]. Present at relatively low abundance in the Bti crystal [18], Cyt2Ba exhibited activity against
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