Potential of bacteria consortium as growth controller of pathogenic fungi Fusarium oxysporum F. sp. cubense (Foc)

Abstract

Utilization of rhizosphere bacteria as agents for controlling of soilborne pathogenic soil microbiomes has been reported in many studies since decades. The aim of the research is identification and characterization the potential of isolated bacteria consortium for controlling of Fusarium oxysporum f.sp. cubense (Foc). The bacteria were selected through the antagonistic test, pathogenic test, and compatibility test. The effectiveness of inhibition of selected bacteria were tested in-vitro through the greenhouse-scale of suppressive soil experiments. The result showed that the Bacillus cereus strain CCM 2010 and BS 3–4B have the highest percentage of inhibition to the Foc (25.68–29.02%). Non-pathogenic bacteria with a percentage of inhibition above 20%, consists of 3 consortia by compatibility testing. Three bacterial consortiums were obtained, the first consortium (BS 3–4B, Bacillus cereus strain CCM 2010, Staphylococcus arlettae strain ATCC 43957, Bacillus cytotoxicus strain NVH 391–98 and Bacillus pseudomycoides strain NBRC 101232), 2nd consortium (Bacillus cereus strain CCM 2010 and Lysinibacillus xylanilyticus strain XDB9), and 3rd consortium (Lysinibacillus xylanilyticus strain XDB9 dan Bacillus pseudomycoides strain NBRC 101232). The data showed application of selected single bacteria able to delay fusarium disease in banana plants more than 45 days post infection in comparison to control plants.