Abstract
Background: Oral lichen planus (OLP) is a T-cell-mediated chronic inflammatory disorder and precancerous oral lesion with high incidence. The current diagnostic method of OLP is very limited and metabolomics may provide a new approach for quantitative evaluation. Methods: The Ultra-Performance Liquid Chromatography-Quadrupole/Orbitrap High Resolution Mass Spectrometry (UHPLC-Q-Orbitrap HRMS) was applied to analyze the change of metabolites in serum of patients with OLP. A total of 115 OLP patients and 124 healthy controls were assigned to either a training set (n = 160) or a test set (n = 79). The potential biomarkers and the change of serum metabolites were profiled and evaluated by multivariate analysis. Results: Totally, 23 differential metabolites were identified in the training set between OLP group and healthy group. Three prominent metabolites in receiver operating characteristic (ROC) were selected as a panel to distinguish OLP or healthy individuals in the test set, and the diagnostic accuracy was 86.1%. Conclusions: This study established a new method for the early detection of OLP by analyzing serum metabolomics using UHPLC-Q-Orbitrap HRMS, which will help in understanding the pathological processes of OLP and identifying precancerous lesions in oral cavity.
Highlights
Oral lichen planus (OLP) is a T-cell-mediated chronic inflammatory disorder and potentially precancerous oral lesion, with a prevalence of 0.5–3% (Warnakulasuriya et al, 2007; Carbone et al, 2009; Kountakis, 2013)
There were more cases had the habit of smoking (p < 0.001) and a preference for very hot food in the OLP group compared with the healthy subjects (p < 0.001)
There was no significant difference among OLP and healthy controls in terms of the habit of drinking (p 0.150) and the preference for spicy food (p 0.330)
Summary
Oral lichen planus (OLP) is a T-cell-mediated chronic inflammatory disorder and potentially precancerous oral lesion, with a prevalence of 0.5–3% (Warnakulasuriya et al, 2007; Carbone et al, 2009; Kountakis, 2013). About 0.5–12.5% of patients with OLP will progress to malignant transformation (Kountakis, 2013). The diagnosis of OLP is usually based on dental exam, histological examinations and patient history (Alrashdan et al, 2016; Raj and Patil, 2017). Oral lichen planus (OLP) is a T-cell-mediated chronic inflammatory disorder and precancerous oral lesion with high incidence. The current diagnostic method of OLP is very limited and metabolomics may provide a new approach for quantitative evaluation
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
