Potential mechanism of biochar enhanced degradation of oxytetracycline by Pseudomonas aeruginosa OTC-T

Abstract

Extensive use of oxytetracycline (OTC) and the generation of its corresponding resistance genes have resulted in serious environmental problems. Physical-biological combined remediation is an attractive method for OTC degradation because of its high remediation efficiency, stability, and environmental friendliness. In this study, an effective OTC-degrading strain identified as Pseudomonas aeruginosa OTC-T, was isolated from chicken manure. In the degradation experiment, the degradation rates of OTC in the degradation systems with and without the biochar addition were 92.71–100 % and 69.11–99.59 %, respectively. Biochar improved the tolerance of the strain to extreme environments, and the OTC degradation rate increased by 20.25 %, 18.61 %, and 13.13 % under extreme pH, temperature, and substrate concentration conditions, respectively. Additionally, the degradation kinetics showed that biochar increased the reaction rate constant in the degradation system and shortened the degradation period. In the biological toxicity assessment, biochar increased the proportion of live cells by 17.63 % and decreased the proportion of apoptotic cells by 58.87 %. Metabolomics revealed that biochar had a significant effect on the metabolism of the strains and promoted cell growth and reproduction, effectively reducing oxidative stress induced by OTC. This study elucidates how biochar affects OTC biodegradation and provides insights into the future application of biochar-assisted microbial technology in environmental remediation.