Abstract
Objective: Mulberry leaf contains Vitamin C, Flavonoids, Oxyresveratrol and Saponin, which accelerated reepithelialization and increases wound contraction in burn healing. This study was designed to asses the effect of mulberry leaf extract ointment (Morus Alba L) on number of fibroblast and collagen fraction area in second degree burns.Materials and methods: This was an experimental design for 6 days with 24 male in Sprague dawley rat randomly selected and divided into 4 groups. The back of Sprague dawley male rats were shaved and given burns using 4 x 2cm iron plate which was put in 98o boiling water for 5 minutes and pasted for 10 seconds and smeared with distilled water, and ointment of mulberry leaf extract. Group I was a positive control (silver sulfadiazide ointment), Group II was a negative control (ointment base), Group III (mulberry leaf extract ointment 10%) and Group IV (mulberry leaf extract ointment 20%). The number of fibroblasts and collagen was evaluated with staining of hematoxylin eosin and staining mallory trichrome.Result and discussion: The mean of fibroblasts I, II, III,and IV was 21.33 ± 1.74, 13.28 ± 1.48, 17.56 ± 1.75, and 23.11 ± 2.38, respectively. The mean collagen fraction area I, II, III, and IV was 21, 11±7,42 , 16,18 ±7,72, 26,078 ± 8, 00 , and 25,79±8,33 respectively. One way Anova followed LSD test showed significant different in mean of between each treatment group obtained significant differences (p <0.05). The mean number of fibroblast in the 10% and 20% ointment extract were significantly different compared with negative controls. Collagen fraction area in treatment group with 10 and 20% ointment were significantly different from that of negative control group, and was not significantly different from silver sulfadiazine groupConclusion: The mulberry leaf extract can be an alternative treatment for second degree burnsInternational Journal of Human and Health Sciences Vol. 05 No. 02 April’21 Page: 235-239
Highlights
IntroductionBurns is one of the most common causes of pain and death in the world. WHO data showed that as many as 265,000 people died every year due to burns
Fibroblasts plays a major role in wound healing reconstruction phase in producing collagen, elastin, bicarbonic acid, fibronectin, and proteoglican[1] Alternative therapy of silver sulfadiazine that has been studied is mulberry leaves (Morus alba L.)
The average amount of fibroblasts and the percentage of collagen in each group are presented in the following figure: The average quantity of fibroblast in the positive control group of 10% mulberry ointment and 20% mulberry ointment was significantly different compared to the negative control group
Summary
Burns is one of the most common causes of pain and death in the world. WHO data showed that as many as 265,000 people died every year due to burns. Former research about mulberry leaves extract showed 20% mulberry leaves cream able to accelerate the healing of second-degree burn wound. It was indicated by re-epithelialization and increasing of wound contraction[2]. There is no research yet about the effect of mulberry (Morus alba L.) leaves extract based cream to the percentage of collagen in the second-degree burn wound healing. Flavonoid on mulberry leaves can reduces the inflammatory process by inhibiting prostaglandins that formed by arachidonic acid and other inflammatory mediators such as histamine and serotonin. It is because they are selective COX-
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