Abstract
Spermicides are substances that can kill spermatozoa. Spermicide circulating in the community contains Nonoxynol9 which, if used for a long time, can irritate the vaginal mucosa and penis. Indonesia has a variety of plants that have the potential to act as anti-fertility, including Sapindus rarak, Centella asiatica, and Azadirachta indica. Objective: This study aim to evaluate extract Sapindus rarak, Centella asiatica, and Azadirachta indica. and mixing of third etract to motility and viability of spermatozoa Guinea pigs. Methods: The sample of this research was 15 guinea pig spermatozoa, 3-4 months old male guinea pigs taken from the cauda epididymis and vas deferens. Guinea pigs were divided into 5 group treatments with each group treatment incubated for 1, 10, and 30 minutes and repeated each for 5 times. The treatments in this study is control, 200 μl of spermatozoa suspension + 50 μl of Sapindus rarak fruit pulp extract 0.9%; 200 μl of spermatozoa suspension + 50 μl of gotu kola extract 0.9%; 200 μl of spermatozoa suspension + 50 μl of 0.9% neem seed extract; 200 μl of spermatozoa suspension + 50 μl of the third mixture extract 0.9%. This study used a completely randomized design (CRD). The observations in this study were the motility of guinea pig spermatozoa categories a + b and the viability of guinea pig spermatozoa using a microscope. The data obtained were analyzed statistically using One Way ANOVA. The results of this study indicated that the administration of extract Sapindus rarak, Centella asiatica, and Azadirachta indica and a mixture of the three extracts had a significant effect (P < 0.05) on the motility of guinea pig spermatozoa categories a + b and viability of guinea pig spermatozoa. Conclution: This study concludes that the mixture of the three extracts has more potential in reducing the motility of spermatozoa categories a + b and the viability of guinea pig spermatozoa compared to single extracts. The mixture of the three extracts may contribute for reduce quality of sperm.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have