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Abstract
Xylanases activity (XY) from Aspergillus japonicus URM5620 produced by Solid-State Fermentation (SSF) of castor press cake (Ricinus communis) on different conditions of production and extraction by PEG/citrate aqueous two-phase system (ATPS) were investigated. XY production was influenced by substrate amount (5–10 g), initial moisture (15–35 %), pH (4.0–6.0) and temperature (25–35 °C), obtaining the maximum activity of 29,085 ± 1808 U g ds−1 using 5.0 g of substrate with initial moisture of 15 % at 25 °C and pH 6.0, after 120 h of fermentation. The influence of PEG molar mass (1000–8000 g mol−1), phase concentrations (PEG 20.0–24.0 % w/w and sodium citrate 15–20 % w/w) and pH (6.0–8.0) on partition coefficient, purification factor, yield and selectivity of XY were determinate. Enzyme partitioning into the PEG rich phase was favored by M PEG 8000 (g mol−1), C PEG 24 % (w/w), C C 20 % (w/w) and pH 8.0, resulting in partition coefficient of 50.78, activity yield of 268 %, 7.20-fold purification factor and selectivity of 293. A. japonicus URM5620 has a potential role in the development of a bioprocess for the XY production using low-cost media. In addition, the present study proved it is feasible to extract xylanase from SSF by adopting the one step ATPS consisting of PEG/citrate.
Highlights
With the increasing concern to valorize the waste material constantly generated, there is considerable interest in the establishment of efficient processes to obtain valuable products from residues that permit the recovery of commercially attractive products from a wide variety of residues (Soccol et al 2011)
Xylanases activity (XY) from Aspergillus japonicus URM5620 produced by Solid-State Fermentation (SSF) of castor press cake (Ricinus communis) on different conditions of production and extraction by PEG/ citrate aqueous two-phase system (ATPS) were investigated
Maximum XY activity of 29,085 ± 1808 U g ds-1 was produced in the culture with 120 h of fermentation, using 5.0 g of substrate with initial moisture of 15 % at 25 °C and pH 6.0
Summary
With the increasing concern to valorize the waste material constantly generated, there is considerable interest in the establishment of efficient processes to obtain valuable products from residues that permit the recovery of commercially attractive products from a wide variety of residues (Soccol et al 2011). The potential production and recovery of enzymes from microbial using cheap agricultural by-products such as castor press cake (Ricinus communis) improve the commercial feasibility of bioprocess technology. Xylan is the second most abundant polysaccharide in nature and it is the major hemicellulosic polysaccharide of wood and agricultural wastes, where it comprises up to 20–35 % dry weight. Considering the nutritional composition of castor press cake, commercial applications for such a valuable material, hitherto, considered a waste in most developing countries need to be explored and one such application could be their use as a substrate for xylanase enzyme production. The design of efficient production and recovery protocols that allow the use of raw material to enzymes production is one of the key goals in the field
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