Abstract
It is essential to develop a simple and sensitive method to rapidly detect residual fungicides in agricultural products to protect human health. So far, little studies have been reported on potential application of gold nanospheres (AuNSps) as a surface plasmon resonance based sensor for in-situ detection of residual fungicides. Therefore, in this study, we investigated the potential application of AuNSps as a surface plasmon resonance based sensor for in-situ detection of fungicides. AuNSps were successfully synthesized via a seed-mediated method with some modifications. Firstly, gold nanoseeds were made during the reduction of chloroauric acid by trisodium citrate dihydrate (TSC). Then, AuNSps were grown from the seeds by using HAuCl4, TSC and EDTA. AuNSps were subsequently dropped on a glass substrate before covered by thiophanate methyl, a broad-spectrum systemic fungicide. The AuNSps coated glass substrate was subsequently dried in the air for further surface-enhanced Raman spectroscopy (SERS) measurements. Optical properties, shape and size of AuNSps were confirmed by UV-vis spectroscopy, XRD, SEM-EDX and TEM. The results showed that AuNSps were successfully synthesized with the size of 53 nm, and their resonance peak was located at 560 nm. The Raman signal intensity of thiophanate methyl covered on AuNSps is higher than that without AuNSps, indicating SERS effects of AuNSps deposited glass substrate.
Highlights
The development of science and technologies has led to the introduction of modern analytical methods with high sensitivity and accuracy, such as high performance liquid chromatography (HPLC), fluorescence assay (FL), high performance liquid chromatography mass spectrometry/mass spectrometry (HPLC–MS/MS), electrochemical assay (EC) and gas chromatography/mass spectrometrySensors 2020, 20, 2229; doi:10.3390/s20082229 www.mdpi.com/journal/sensorsSensors 2020, 20, x FOR PEER REVIEWSensors 2020, 20, 2229 spectrometry (GC/MS)
Raman spectra of thiophanate methyl were coated recorded on substrate, an Au nanospheres (AuNSps) coated glasswas substrate rinsed a 3:1 (v/v) mixture of concentrated
Raman peaksInterestingly, of thiophanate methyl clearly observed after themethyl, deposition methyl onto peaks of thiophanate methyl were clearly observed after the deposition ofofthiophanate methyl onto the AuNSps coated glass substrate
Summary
The development of science and technologies has led to the introduction of modern analytical methods with high sensitivity and accuracy, such as high performance liquid chromatography (HPLC), fluorescence assay (FL), high performance liquid chromatography mass spectrometry/mass spectrometry (HPLC–MS/MS), electrochemical assay (EC) and gas chromatography/mass spectrometry. Gold nanoparticles with non-toxicity and high bioactivity applications modern medical and biological studies suchofasapplications drug, DNAinand antigens delivery; have gained in much interest, and they present a wide range modern medical and biosensorics, genomics, immunoanalysis, detection of microorganisms and cancer cells and biological studies such as drug, DNA and antigens delivery; biosensorics, genomics, immunoanalysis, bioimaging. Gold nanoparticles of various sizes, shapes and structures such as nanospheres, nanorods, Thiophanate methyl whose chemical structure is shown in Figure is known as a member of the nanoshells, nanocages, nanostars, etc. Assessment exposure thiophanate methylitin develop a sensitive on-site method to detect residual thiophanate methyl in agricultural products to products may cause serious effects on human health [16]. Little studies have been reported on the potential nanospheres (AuNSps)—supported Raman spectroscopy—has the potential to be an accurate portable application of AuNSps aslittle a surface plasmon resonance based sensor for in-situ detection of residual analytical method.
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