Abstract
Routine laboratory lipid assays include simple measurements of total cholesterol, triacylglycerols and HDL. However, lipids are a large group of compounds involved in many metabolic pathways, and their alterations may have serious health consequences. In this study, we used 1H NMR to analyze lipids extracted from sera of 16 obese patients prior to and after bariatric surgeries. We observed a post-surgery decrease in serum concentrations of lipids from various groups. The hereby presented findings imply that 1H NMR is suitable for rapid, simple and non-invasive detection of lipids from 30 structural groups, among them triacylglycerols, phosphatidylcholine, phosphatidylethanolamine, sphingomyelin, total phospholipids, total, free and esterified cholesterol, total and unsaturated fatty acids. NMR-based analysis of serum lipids may contribute to a substantial increase in the number of routinely determined markers from this group; therefore, it may find application in clinical assessment of obese subjects prior to and after bariatric surgeries, as well as in the examination of patients with other metabolic diseases.
Highlights
Diseases is still not fully understood[17]
We still lack an accurate method for rapid determination of whole spectrum of blood lipids. 1H NMR may be helpful in early detection of disorders in various groups of serum lipids being associated with a plethora of diseases, among them obesity
We showed that extraction with chloroform and methanol mixture, followed by NMR analysis lasting no longer than a few minutes, is a time-sparing method that can detect up to 10 various groups of lipids, including TG, PC, PE, SM, total PL, total fatty acids and unsaturated fatty acids (UFA), as well as total, free and esterified cholesterol
Summary
Diseases is still not fully understood[17]. Changes in polar lipid profile are observed during the course of many metabolic diseases, including obesity, and may be reflected by altered properties of plasma membranes[17]. The aim of this study was to analyze the impact of bariatric surgery on the levels of lipids from selected groups being vital for health of obese patients. To achieve this goal, we used a new approach, NMR spectroscopy, as this method offers many advantages which are not available in the case of conventional laboratory techniques. The study included patients with morbid obesity, examined prior to and after bariatric surgery; our hereby presented diagnostic method is likely applicable to other metabolic diseases. We were able to detect some specific lipid compounds, such as 7-lathosterol, oleic acid and linoleic acid
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