Abstract
This study was conducted in stone fruit orchards in five provinces of the East Mediterranean Region of Turkey between the years of 2009-2011. The aim of the study was to determine the potential aphid vectors of the quarantine pathogen PPV (plum pox virus, family Potyviridae) that causes serious yield losses. During the surveys, 542 orchard/nurseries were sampled and 6 aphid species (Hemiptera: Aphididae) [Brachycaudus persicae (Passerini,1860), B. helichrysi (Kaltenbach, 1843), B. cardui (Linnaeus, 1758), Hyalopterus pruni (Geoffroy, 1762), Myzus persicae (Sulzer, 1776) and M.cerasi (Fabricius, 1775)] from stone fruit trees and 4 aphid species [Aphis craccivora (Koch, 1854), A. fabae Scopoli 1763, A. gossypii Glover 1877, A. nasturtii Kaltenbach 1843)] from weeds that were known as the efficient vectors of PPV were detected in the stone fruit orchards. Stone fruit samples (flower, leaf, fruit), weed samples and aphid samples were tested by DAS-ELISA to determine the presence of PPV. PPV infected samples that resulted uncertain from DAS-ELISA were processed to conventional RT-PCR (Reverse Transcription Polymerase Chain Reaction) to finalize the decision of PPV presence. A total of 8 orchards were resulted as PPV-infected with both analyzing methods in the region. Three of these orchards were infected with aphids as well. B.cardui collected from the PPV-infected orchard in Hatay-Samandağ were also run to analyse for the presence of PPV and resulted virus positive.
Highlights
From weeds that were known as the efficient vectors of PPV were detected in the stone fruit orchards
Aphids are the most common and efficient vectors owing to piercing-sucking mouthparts that provide transmission of the virus to healthy plants
Hazır et al - Journal of Agricultural Sciences (Tarim Bilimleri Dergisi), 2021, 27(4): 484-492 identified as PPV herbaceous hosts under experimental and under field conditions (Milusheva & Rankova 2002; Viršček 2004; Celetti et al 2008)
Summary
From weeds that were known as the efficient vectors of PPV were detected in the stone fruit orchards. Stone fruit samples (flower, leaf, fruit), weed samples and aphid samples were tested by DAS-ELISA to determine the presence of PPV. A total of 8 orchards were resulted as PPV-infected with both analyzing methods in the region. Three of these orchards were infected with aphids as well. PPV (plum pox virus, family Potyviridae) is not persistent in the aphid and is transferred from the mouthparts of the aphid between plants non-persistently. As well as being a possible reservoir of PPV, weeds play an important role as being host plant for aphid vectors
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