Abstract
Thyroid cancer is one of the most common types of cancer in endocrine system. In latest studies, harmine has been proved to inhibit the growth of several cancers in vitro and in vivo. In the current study, we evaluated the in vitro and in vivo anticancer efficiency of harmine against thyroid cancer cell line TPC-1. The in vitro cytotoxicity of harmine evaluated by XTT assay indicated that harmine suppressed the proliferation of TPC-1 cells in a dose- and time-dependent manner. Moreover, harmine dose-dependently induced apoptosis of TPC-1 cells through regulating the ratio of Bcl-2/Bax. The colony forming ability of TPC-1 cells was also time-dependently inhibited by harmine. The inhibitory effects of harmine on migration and invasion of TPC-1 cells were studied by wound scratching and Transwell assays. In vivo evaluation showed that harmine effectively inhibited the growth of thyroid cancer in a dose-dependent manner in nude mice. Therefore, harmine might be a promising herbal medicine in the therapy of thyroid cancer and further efforts are needed to explore this therapeutic strategy.
Highlights
Thyroid cancer is one of the most common kinds of cancer in endocrine system which includes four major types with papillary thyroid cancer (PTC) being the most common one [1]
Human papillary thyroid cancer cell line TPC-1 was purchased from the Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences (Shanghai, China)
Harmine inhibited the proliferation of TPC-1 cells in a time- and dose-dependent manner
Summary
Thyroid cancer is one of the most common kinds of cancer in endocrine system which includes four major types with papillary thyroid cancer (PTC) being the most common one [1]. A growing body of evidences demonstrated the promising antitumor efficiency of herbal medicine [3,4,5]. Several herbal medicines, such as harmine, resveratrol, and berbamine, have shown substantial tumor inhibitory effect against a series of cancers [6,7,8]. Possible mechanisms include the block of homologous recombination, one of the major Double Strand Break (DSB) repair pathways and the inhibition of telomerase activity [9, 12]. No related reports were found on the antitumor effect of harmine in thyroid cancer
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