Potential Antioxidant and Anti-Inflammatory Function of Gynura procumbens Polyphenols Ligand.

Hun Hwan Kim,Preethi Vetrivel,Seong Min Kim,Gon Sup Kim,Pritam Bhagwan Bhosale,Sang Eun Ha

doi:10.3390/ijms22168716

Hun Hwan Kim, Preethi Vetrivel + Show 4 more

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https://doi.org/10.3390/ijms22168716

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Abstract

The antioxidant and anti-inflammatory potentials of polyphenols contained in Gynura procumbens (GP) extract were systematically analyzed. Polyphenols in GP were analyzed for nine peaks using high-performance liquid chromatography (HPLC) combined with mass spectrometry (MS), and quantitatively determined through each standard. A total of nine polyphenolic compounds were identified in the samples and their MS data were tabulated. To determine the potential of bioactive ingredients targeting DPPH and COX-2, we analyzed them by ultrafiltration combined with LC. The results identified the major compounds exhibiting binding affinity for DPPH and COX-2. Caffeic acid, kynurenic acid, and chlorogenic acid showed excellent binding affinity to DPPH and COX-2, suggesting that they can be considered as major active compounds. Additionally, the anti-inflammatory effect of GP was confirmed in vitro. This study will not only be used to provide basic data for the application of GP to the food and pharmaceutical industries, but will also provide information on effective screening methods for other medicinal plants.

Highlights

It is known that diseases caused by oxidative and inflammatory stress are largely contributed to by prostaglandins and free radicals produced during metabolic processes in the human body [1]
This study confirmed the action mechanism of the compounds contained in Gynura procumbens simultaneously using DPPH–high-performance liquid chromatography (HPLC) and bio-affinity UF-HPLC method
Through LC-mass spectrometry (MS)/MS, nine compounds contained in the polyphenol extract of Gynura procumbens were identified and quantified, and the reaction amount and reaction rate (%) were confirmed to effectively work with the main compounds used for antioxidant and antiinflammatory activity

Summary

Introduction

It is known that diseases caused by oxidative and inflammatory stress are largely contributed to by prostaglandins and free radicals produced during metabolic processes in the human body [1]. Because such stress contributes to the onset and progression of various chronic diseases, such as cancer, liver disease, and autoimmune diseases [2], research to overcome it continues to this day. Synthesized drugs are most commonly used to treat disease [3], but long-term use causes side effects on the liver, kidneys, and organs in the body [4]. Plant-based materials have been used as a dietary supplements or medicine based on their various activities [15]

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