Abstract
Purpose : To evaluate the acticonvulsant activity of Cichorium intybus ( C. intybus ) and Taraxacum serotinum ( T. serotinum ) in maximal electroshock (MES), as well as pentylenetetrazole (PTZ)- and strychnine nitrate (STN) - induced seizure models in rats. Methods : For each model, 8 groups of Swiss albino rats (n=10) were used. The 1st group was kept as control, 2nd as standard (diazepam, 7.5 mg/kg); 3rd - 5th were treated with C. intybus ethanol extract (125, 250 and 500 mg/kg); and 6th - 8th treated with T. serotinum extract (125, 250 and 500 mg/kg). After 30 min of administration, the rats were exposed to a shock of 150 mA by a convulsiometer, via ear electrodes for 2 s (in MES test) or sc injection of PTZ (85 mg/kg) or STN (2.5 mg/kg). Anticonvulsant activity was confirmed by abolition of hind limb tonic extension (HLTE) in MES test and by measuring the latency to PTZ or STN-induced threshold seizures, and the duration of seizures in the rats. Results : In MES model, 500 mg/kg of C. intybus and T. serotinum resulted in complete abolition of HLTE in 70 and 50 % of the rats, respectively, compared to 80 % in diazepam-medicated animals. Both extracts at 500 mg/kg prolonged latency to seizure onset in PTZ model to 144.7 and 114.7 s, respectively (vs 55.2 s in control group; p < 0.05). Both extracts failed to protect rats against STNinduced seizures. Conclusion : C. intybus and T. serotinum possess anticonvulsant effect as they both abolish HLTE induced by MES and delay the latency of seizures produced by PTZ. Keywords : Cichorium intybus , Taraxacum serotinum , Anticonvulsant, Seizures, Maximal electroshock, Pentylenetetrazole, Strychnine nitrate
Highlights
Despite fundamental progress made in the treatment of neurological disorders, epilepsy remains a significant therapeutic defiance
The results obtained indicated that C. intybus and T. serotinum extracts at oral doses up to 5000 mg/kg did not produce any symptom of acute toxicity and none of the rats died during 24 h of observation
Treatment of rats with the ethanolic extracts of C. intybus (125 mg/kg) and T. serotinum (125 and 250 mg/kg), did not produce significant effect against seizure induced by maximal electroshock seizure (MES)
Summary
Despite fundamental progress made in the treatment of neurological disorders, epilepsy remains a significant therapeutic defiance. Experimental doses of 125, 250 and 500 mg/kg that are equal to 1/40, 1/20 and 1/10 of the highest possible dose tolerated by rats were selected for the study Both the electrically induced seizure model (MES) and chemically induced seizure models (scPTZ and scSTN) were used to determine the anticonvulsant activity of C. İntybus and T. serotinum extracts in 21-day-old Albino rats. Groups VI, VII and VIII received T. serotinum extract at oral doses of 125, 250 and 500 mg/kg, respectively. On the 10th day, 30 min after administration of the last dose of the vehicle, diazepam and the test extracts, seizures were induced in rats by subcutaneous injection of PTZ (85 mg/kg, for PTZ-induced seizure test)or STN (2.5 mg/kg, for STN-induced seizure test).
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