Potential and safety tests of egg drop syndrome candidate vaccine from Medan isolate, Indonesia

Gusti Ayu Yuniati Kencana,Gusti Ayu Mayani Kristina Dewi,I Made Kardena,Arini Nurhandayani,Syamsidar Syamsidar,Kadek Karang Agustina,Nyoman Suartha

doi:10.14202/vetworld.2018.1637-1640

Gusti Ayu Yuniati Kencana, Gusti Ayu Mayani Kristina Dewi + Show 5 more

Open Access

https://doi.org/10.14202/vetworld.2018.1637-1640

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Journal: Veterinary World	Publication Date: Nov 1, 2018
Citations: 3	License type: CC BY 4.0

Affiliation: Udayana University

Abstract

Aim:The study was aimed to prepare and examine the potential and safety concerns of egg drop syndrome (EDS) vaccine candidate seed. The potential and safety trials of EDS Medan isolate vaccine need to be done before commercial scale of EDS vaccines are made.Materials and Methods:The safety test of EDS candidate vaccine was tested on 4-week-old specified pathogen-free chickens in an experimentally isolated enclosure.Results:The result of the safety test obtained 27.3 hemagglutination inhibition (HI) unit of geometric mean titer antibody post-vaccination. However, the potency test of the EDS candidate vaccine was conducted on 17-week-old laying hens. Test results of the EDS potency vaccine in layer obtained antibody titer increased in every week of blood taking with average titer of antibody: Before vaccinated was 22.9 HI unit, 1 week after vaccination was 23.7 HI unit, 2 weeks post-vaccination was 25 HI unit, and 3 weeks after vaccination was 27.3 HI units. In contrast, decreasing trend was observed in control group (unvaccinated chicken).Conclusion:Serologically, the seed vaccine of EDS virus isolates from Medan was able to produce protective antibody titers starting in the 2nd and 3rd weeks post-vaccination.

Highlights

Egg drop syndrome (EDS) is a disease caused by the duck adenovirus belonging to family Adenoviridae and genus of Atadenovirus
Potency test of EDS vaccine seeds in commercial laying chickens vaccinated at 17 weeks, produced an antibody titer that increased in every week post-vaccination
The EDS vaccine candidate of Medan isolates is eligible for EDS vaccine because it was able to produce antibody titer of 27.3 hemagglutination inhibition (HI) units in layer vaccinated at 17 weeks (Figure-2)

Summary

Introduction

Egg drop syndrome (EDS) is a disease caused by the duck adenovirus belonging to family Adenoviridae and genus of Atadenovirus. The virus was 1st time discovered in 1976 in the Netherlands [1]. It was called EDS - 1976 and later on abbreviated to EDS-76 [2]. Adenovirus particles are icosahedral in structure with 70-90 nm in diameter [3]. Virus particles are composed of 252 capsomers of triangular form. The genome of EDS 1976 is linear and composed of double-stranded deoxyribonucleic acid (DNA). Some strains of adenovirus can survive at 60-70°C for 30 min. The F1 strain can survive up to 56°C for 18 h. In Group I adenovirus, only F1 strain can agglutinate the red blood cells [4,5]

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