Potent Antiproliferative Effect on Liver Cancer of Medicinal Plants Selected from the Thai/Lanna Medicinal Plant Recipe Database "MANOSROI III".

Aranya Manosroi,Worapaka Manosroi,Worapong Kitdamrongtham,Jiradej Manosroi,Hiroyuki Akazawa,Toshihiro Akihisa

doi:10.1155/2015/397181

Abstract

Thai/Lanna medicinal plant recipes have been used for the treatment of several diseases including liver cancer. In this study, methanolic extracts (MEs) of 23 plants were tested for antiproliferative activity on human hepatoma cell line (Hep G2) by the sulforhodamine B (SRB) assay. Nine MEs with potent antiproliferative activity (IC50 < 100 µg/mL) were obtained and further semipurified by liquid/liquid partition extraction. The semipurified fractions were tested for the antiproliferative and antioxidative activities. ME of Stemona collinsae and the semipurified extract and methanol-water fraction (MF) of Gloriosa superba gave the highest antiproliferative activity on HepG2 which were 4.79- and 50.07-fold cisplatin, respectively. The semipurified fractions showed an increased antiproliferative activity. MF of Caesalpinia sappan and HF of Senna alata showed the highest free radical scavenging and metal chelating activities, respectively. The compound in n-hexane fraction (HF) of Ventilago denticulata which showed an increase in antiproliferative activity comparing to its ME was isolated and identified as emodin. This study has demonstrated the potential of the ME from S. collinsae, MF from G. superba, and emodin isolated from V. denticulata, for further development as an antiliver cancer agent.

Highlights

Hepatocellular cancer and intrahepatic cholangiocarcinoma are the two major forms of primary liver cancers
methanolic extracts (MEs) of S. collinsae showed A activity with the highest antiproliferative activities (IC50 = 5.51 μg/mL) on HepG2 cell line which was 4.79- and 0.10-fold cisplatin and doxorubicin, Table 1: Botanical names, families, part used, yields, and antiproliferative activity on human hepatoma cell line (HepG2) of the 23 methanol extracts of the medicinal plant selected from Thai/Lanna medicinal recipe database “MANOSROI III.”
The antiproliferative activity on Hep G2 cell line of the 23 medicinal plants selected from the “MANOSROI III” database was investigated

Summary

Introduction

Hepatocellular cancer and intrahepatic cholangiocarcinoma are the two major forms of primary liver cancers. The incidence of these two carcinomas is the fifth most common widespread cancer in the world. Several types of chemotherapeutic drugs have been used to treat hepatic cancer such as cisplatin, 5-fluorouracil, and paclitaxel. These drugs usually have some problems of cancer resistance, due to multidrug resistance protein and the decrease of apoptotic proteins. Most medicinal plants from traditional medicines are safer than the chemical substances with effectiveness. This may be due to the traditional uses in human for several generations. Those which showed therapeutic efficacy and safety will be recorded and used until now

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