Potent Affinity Labeling Peptide Inhibitors of Calpain

Abstract

Abstract We have employed a recently described affinity labeling approach using S-(3-nitro-2-pyridinesulfenyl) group (Npys) to devise unique inhibitors of the Ca++-dependent thiol protease, calpain. Compounds with Npys group bound on the peptides with Leu-Leu or Val-Val sequences were found to be highly potent inhibitors of calpain.