Abstract
Antibacterial compounds are compounds that can inhibit bacterial growth. The components contained in the basil leaf extract have the potential to be developed as a source of antibacterial active ingredients. Basil leaves are a plant that has antibacterial potential. This study aims to test the antibacterial activity of the basil leaf extract (Ocimum sanctum L.) with various concentrations of 20%, 40%, 60%, 80%, and 100% against Staphylococcus aureus ATCC 25923. Antibacterial activity test using the well diffusion method. The results showed that basil leaf extract (Ocimum sanctum L.) concentrations of 20%, 40%, 60%, 80%, and 100% could inhibit the growth of Staphylococcus aureus ATCC 25923. The concentration of basil leaf extract was 100% effective as an antibacterial against Staphylococcus aureus bacteria. ATCC 25923 of 12.60 mm.
Highlights
Senyawa antibakteri merupakan senyawa yang dapat menghambat pertumbuhan bakteri
This study aims to test the antibacterial activity of the basil leaf extract (Ocimum sanctum L.) with various concentrations of 20%, 40%, 60%, 80%, and 100% against Staphylococcus aureus ATCC 25923
The results showed that basil leaf extract (Ocimum sanctum L.) concentrations of 20%, 40%, 60%, 80%, and 100% could inhibit the growth of Staphylococcus aureus ATCC 25923
Summary
Alat yang digunakan dalam penelitian ini yaitu timbangan analitik, oven, autoclave, Laminar Air Flow, waterbath, shaker, inkubator, petridish, pinset, botol vial, bunsen, hot plate, magnetic stirrer, palu sumuran, jangka sorong, mikropipet, tip pipet, erlenmeyer, dan Mc. Farland Standart. Bahan Ekstrak kemangi, bakteri Staphylococcus aureus diperoleh dari Balai Laboratorium Kesehatan Yogyakarta. Media yang digunakan meliputi Nutrient Agar untuk peremajaan bakteri uji, media Muller Hinton Agar untuk uji aktivitas antibakteri. Bahan lainnya meliputi alkohol 70%, chlorampenicol, aquadest, swab steril, dan paperdisk. Alat Alat yang digunakan dalam penelitian ini yaitu timbangan analitik, oven, autoclave, Laminar Air Flow, waterbath, shaker, inkubator, petridish, pinset, botol vial, bunsen, hot plate, magnetic stirrer, palu sumuran, jangka sorong, mikropipet, tip pipet, erlenmeyer, dan Mc. Farland Standart
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