Abstract
Begonia hirtella leaf are commonly used by people in mountainous areas to treat itchy due to insect bites and skin infection. This study aims to (1) determine proper solvent extract of B. hirtella leaf on antibacterial activity of S. aureus and E. coli and on antifungal activity of C. albicans (2) determine the lowest concentration of leaf extract of B. hirtella that indicate the formation of inhibition zone (3) know the content of bioactive compounds contained in each leaf extract of B. hirtella. The method used in this study is experimental with completely randomized design (CRD). The treatments were type of solvents (n-hexane, ethyl acetate, ethanol and water) and a serie of the ethyl acetate extract concentration of 500 ppm, 450 ppm, 400 ppm, 350 ppm, 300 ppm and 250 ppm against S. aureus, E. coli and C. albicans. Data were analyzed using analysis of variance (Anova) and the significant differences between the treatments were analyzed by Duncan test at 95% confidence level. The result showed that each solvent extracts affect microbes growth with a highly significant difference p <0.05. Solvent extracts that have the best inhibitory zone is ethyl acetate against S. aureus with an average diameter of inhibitory zone 13.75 ± 1.26 mm. Increasing concentrations of ethyl acetate extract of 250 ppm to 500 ppm increase inhibition zone against microbes. The lowest concentration that show inhibition zone was 300 ppm. The formation inhibition of zone on microbes growth happened due to their compounds in the extract. Extract of n-hexane contains stigmasterol, ethyl acetate contains neophytadiene, while the ethanol extract contains ethyl palmitate
Highlights
Daun Begonia hirtella biasa digunakan masyarakat di daerah pegunungan untuk mengobati gatal karena digigit serangga dan gatal karena infeksi kulit
This study aims to (1) determine proper solvent extract of B. hirtella leaf on antibacterial activity of S. aureus and E. coli and on antifungal activity of C. albicans (2) determine the lowest concentration of leaf extract of B. hirtella that indicate the formation of inhibition zone (3) know the content of bioactive compounds contained in each leaf extract of B. hirtella
Uji Aktivitas Antibakteri Ekstrak Kulit Buah Tampoi (Baccaurea macrocarpa) terhadap Bakteri Escherichia coli dan Staphylococcus aureus
Summary
Dibuat seri larutan standar asam tanat dalam etanol yang digunakan sebagai kurva standar. Pembuatan standar asam tanat yaitu melarutkan asam tanat dalam etanol 95%, kemudian dibuat seri pengenceran 0 ppm, 25 ppm, 50 ppm, 75 ppm, 100 ppm, dan 125 ppm. Pengenceran dan dimasukkan ke dalam tabung reaksi bersih. Ditambah 1 ml etanol 95%, 5 ml air bebas ion dan 0,5 ml reagen folin ciocalteau 50% pada masing-masing tabung reaksi. Setelah 5 menit ditambah 1 ml larutan Na2CO3 5%, kemudian divortex dan disimpan pada tempat gelap selama 60 menit. Setelah diinkubasi pada tempat gelap, sampel divortex kembali dan diukur absorbansinya pada panjang gelombang 725 nm. Alat ini digunakan untuk mengetahui jenis dan kadar senyawa bioaktif dari ekstrak daun B. hirtella
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