Potency of Nanopropolis Stinglessbee Trigona spp Indonesia as Antibacterial Agent

Abstract

Propolis is one of the natural produced by honey bee which have many benefits, due to its properties as antibacterial, antivirus, and anticancer. The aim of this study is to determine potency of nanopropolis honey bee from Pandeglang Banten Indonesia as antibacterial agent. Encapsulated nanopropolis was prepared by high speed homogenization technique followed by encapsulation using maltodekstrin with solvent evaporation technique. The size of nanopropolis was measured with SEM. The result of SEM show that size of nanopropolis is 175 nm. The antibacterial activity test was performed by agar well diffusion. Nanopropolis have activtiy as antibacterial agent for Gram positive and Gram negative bacteria. The nanopropolis showed higher bacterial activity than extract propolis were 205.86% for B. subtilis, 211.83% for S. aureus, 227.01% for E. coli, and 230.29% for Salmonella sp. While compared to amphicillin 10 mg/ml equel to 43.87%, 49.12%, 42.35%, and 37.58%. Minimum Inhibitory Concentration (MIC) of nanoproplis were 0.313%, 0.15%, 0.313%, and 0.625% for B. subtilis, S. aureus, E.coli, dan Salmonella sp respectively.