Abstract
BackgroundThe Plutella xylostella PxSDF2L1 gene was previously reported to enhance insect resistance to pathogen at high basal transcription rate. PxSDF2L1 shows similitude with the stromal cell-derived factor 2 (SDF2), an ER stress-induced chaperon protein that is highly conserved throughout animals and plants. The precise biological function of SDF2 is not clear, but its expression is required for innate immunity in plants. Here, we investigate whether a continuous expression of PxSDF2L1 in Nicotiana benthamiana can similarly confer resistance to plant pathogen, particularly, the black shank Phytophthora parasitica var. nicotianae.ResultsThe N. benthamiana plants were inoculated with agrobacteria transformed with a PVX-based binary vector carrying the PxSDF2L1 gene; similar agroinoculation experiments with a PVX vector carrying the GFP gene were used for controls. In pot trials, agroinfected N. benthamiana plants constitutively expressing PxSDF2L1 showed a significant reduction of stem disease symptoms caused by the inoculation with P. parasitica, compared with controls.ConclusionsWe confirm a role of PxSDF2L1 in resistance to black shank, with a potential application to engineering active resistance against this oomycete in the commercial N. tabacum species and propose its evaluation in other crop families and plant pathogens.
Highlights
The Plutella xylostella PxSDF2L1 gene was previously reported to enhance insect resistance to pathogen at high basal transcription rate
Potato virus X (PVX) vector DNA contamination was not PVX.PxSDF2L1-challenged N. benthamiana plants are resistant to P. parasitica infection Nicotiana benthamiana is a host of P. parasitica
PVX.green fluorescent protein (GFP)- and PVX.PxSDF2L1-agroinfected N. benthamiana plants, 21 d.p.ai., were inoculated with the isolate PpnIIT23 of P. parasitica race 0 and disease symptoms scored at 7 days post-inoculation (d.p.i.)
Summary
The Plutella xylostella PxSDF2L1 gene was previously reported to enhance insect resistance to pathogen at high basal transcription rate. PxSDF2L1 shows similitude with the stromal cell-derived factor 2 (SDF2), an ER stress-induced chaperon protein that is highly conserved throughout animals and plants. The precise biological function of SDF2 is not clear, but its expression is required for innate immunity in plants. The stromal cell-derived factor 2 (SDF2) is an endoplasmic reticulum (ER)-resident protein that is highly conserved in plants and animals [1, 2]. SDF2 is part, with ER-resident chaperones Hsp protein ERdj, Hsp luminal binding protein (BiP) and other folding enzymes of the ER quality control (ER-QC) machinery, controlling the folding status of secreted and transmembrane proteins to ensure the delivery of functional molecules to their final destination [4, 5]. SDF2-like 1 (SDF2L1) protein retards the degradation of unfolded proteins by the ER-associated protein degradation (ERAD) machinery [8]
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