Potato phytopathogene bank and in vitro seed farming: illumination, medium

Abstract

The investigation of light spectrum influence on growth and development of potato single node cuttings was conducted. Regionized cultivars were involved into the study. Apical meristems were used as explants for microplants creation and cultivated on Murashige-Skoog medium with adding. Microplants were exposed to three light treatments: red LEDs, blue LEDs and white luminescent lamps. It was shown, that red light is not suitable for potato single node cuttings cultivation in vitro under light intensity of 45 µmol photons/m2s. Red and blue light increased the rate of cuttings rhisogenesis. The maximum of nodes per plant developed under blue and white light. The influence of genotype on node quantity was detected. Tuleevskiy cultivar had maximum nodes per microplant and considers being the most perspective for micropropagation, Favorit cultivar – minimum. Microtuberization was obtained on the node cultivation medium in the presence of 1 mg/l IAA and 0.04 mg/l kinetine. The minimum weight and number of microtubers were appropriate to the samples cultivated on hormonal medium. The screening of potato tubers and plant cuttings, which had been collected across Krasnoyarsk Territory, for phytopathogenes presence, was conducted. Alternaria and Fusarium genera dominated among detected micromycetes. Isolates of Botrytis, Aspergillus, Penicillium, Trichothecium genera were recovered.