Abstract
Ultrathin sections of Araldite- and Maraglas-embedded tissues may be cleanly stained by submersion for 30 minutes in a freshly prepared 1.0% aqueous solution of KMnO4. Results resemble those achieved with lead stains. By submersion of the sections throughout the staining and washing procedure, contamination of the specimen is avoided. Capricious precipitation of the stain, granularity of the section, tissue damage, and fragility of the section in the electron beam (reported disadvantages of KMnO4 as an electron stain) have not been encountered. Destructive effects associated with KMnO4 staining may be due to excessive or indiscriminate use of agents, e.g., citric acid, to remove precipitated stain. Use of such bleaching agents is unnecessary after properly executed staining procedures. The only disadvantage encountered is failure of cytoplasmic RNP particles to stain completely after OsO4 fixation.
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