Potassium Linoleate (Isomerized) Satisfies the United States Environmental Protection Agency MB-05-16 for Hospital Disinfectant on Hard, Non-porous Surfaces.

David G Changaris,Anne L Carenbauer

doi:10.7759/cureus.22851

Abstract

Potassium conjugated linoleic acid or potassium linoleate (isomerized), 86 mM, satisfies the United States Environmental Protection Agency protocol hospital disinfectant for non-porous surfaces MB-05-16 with one-minute treatment. This stringent protocol requires separate preparations of Staphylococcus aureus (American Type Culture Collection 6538) and Pseudomonas aeruginosa (American Type Culture Collection 15442) unstirred for 48 hours, submerging 10 mm polished cylinders in the culture, and placing for 45 minutes in a 37°C humidified chamber before treating. Since potassium linoleate (isomerized) also satisfies the United States Environmental Protection Agency protocol MB-35-00 for Candida auris, this study establishes potassium linoleate (isomerized) as an effective cross-kingdom antimicrobial plant salt, soap, or cleanser. We affirm the need for formal post-treatment plating on agar to establish efficacy and not rely on OD600 when testing for antimicrobial capacity. Aqueous dilution of the soap causes variable opalescence making optical density an unreliable marker for antimicrobial efficacy.

Highlights

Surface disinfection has moved center stage in efforts to reduce hospital-acquired infections (HAI) [1-4]
Abundant naturally occurring enzymes participate in degradation as well as natural oxidative stressors metabolize Conjugated linoleic acid (CLA) [20,21]
Faced with the prospect of changing the hazmat status of the entire building with available respiratory protection protocols and training, we chose to qualify the cylinders with potassium linoleate

Summary

Introduction

Surface disinfection has moved center stage in efforts to reduce hospital-acquired infections (HAI) [1-4]. The observed correlation between drug-resistant bacteria within a hospitalized patient and subsequent infection in another patient who subsequently occupies the same room has increased “turn-over room disinfection” efforts with some success [5-8]. Efforts to reduce the transmission of drug-resistant microbes to the occupant of the room include quaternary amines, bleach, peroxide, peracetic acid, ultraviolet light (ozone), aerosolized disinfectants [7]. Exposing hospital personnel to these disinfectants can cause skin and pulmonary injuries [9-11]. These increasingly toxic procedures require longer recovery times before humans can enter an increasingly exotic biosphere. None of these efforts has resulted in eliminating HAI

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