POTASSIUM HUMATE INFLUENCE FOR THE BROILER CHICKENS ENZYMATIC PROFILE

Abstract

The purpose of research is increase activity of liver enzymes by the use of potassium humate. It was performed the enzyme profile of hepatocytes of broiler chickens with additional load solution of potassium humate. The following indicators: the activity of catalase, super-oksiddismutazy, glutathione peroxidase, alkaline phosphatase, alanine aminotransferase and aspartate aminotransferase, and the number of malondialdehyde were examined. It was established that the enzyme activity increases with age in the control and experimental groups. Thus there is an increase in the experimental activity aspartataninaminotransferaze 35 to 45 days. On 45 day it was reached 16.80 % (P <0.05) in relation to indicators of intact animals. Changes in the activity of alanine aminotransferase, during the experiment was not significant and was 14.21% compared to control the experimental group showed an increase in superoxide dismutase activity in the age range 25-45 days to 11.43% (P <0,05) with respect to intact animals. Activity glutationpirocside a group of poultry, geting potassium humate, above the reference value at 28.74% (P <0.05). Alkaline phosphate activity was increased in the course of the experiment in chickens at the age of 5-30 days, and then observed its decline. Value efficiency AP 45 hours in the experimental group below the control values at 12.82% (P <0.05). The activity of catalase to 13.22% (P <0.05) relative to the control group was decreased. Fluctuations in the on-indicators in the experimental group at the age of 5-45 days slightly was shown. Malone dialdehyde concentration below the control valuetion to 11.40% (P <0.05), this is due to high relative to the control group, the activity of the glutathione peroxidase. Thus, when administered in the diet of experimental, the poultry potassium humate of 45 days there was increased activity of the liver enzymes such as alanine aminotransferase, aspartate aminotransferase, superoxide dismutase, glutathione peroxidase, and reducing the activity of alkaline phosphatase and catalase.