Potassium Fertilization Stimulates Sucrose-to-Starch Conversion and Root Formation in Sweet Potato (Ipomoea batatas (L.) Lam.).

Yang Gao,Shenyuan Pan,Zhonghou Tang,Houqiang Xia,Jingran Liu,Ming Liu,Minfei Sheng,Zongyun Li

doi:10.3390/ijms22094826

Abstract

A field experiment was established to study sweet potato growth, starch dynamic accumulation, key enzymes and gene transcription in the sucrose-to-starch conversion and their relationships under six K2O rates using Ningzishu 1 (sensitive to low-K) and Xushu 32 (tolerant to low-K). The results indicated that K application significantly improved the biomass accumulation of plant and storage root, although treatments at high levels of K, i.e., 300–375 kg K2O ha−1, significantly decreased plant biomass and storage root yield. Compared with the no-K treatment, K application enhanced the biomass accumulation of plant and storage root by 3–47% and 13–45%, respectively, through promoting the biomass accumulation rate. Additionally, K application also enhanced the photosynthetic capacity of sweet potato. In this study, low stomatal conductance and net photosynthetic rate (Pn) accompanied with decreased intercellular CO2 concentration were observed in the no-K treatment at 35 DAT, indicating that Pn was reduced mainly due to stomatal limitation; at 55 DAT, reduced Pn in the no-K treatment was caused by non-stomatal factors. Compared with the no-K treatment, the content of sucrose, amylose and amylopectin decreased by 9–34%, 9–23% and 6–19%, respectively, but starch accumulation increased by 11–21% under K supply. The activities of sucrose synthetase (SuSy), adenosine-diphosphate-glucose pyrophosphorylase (AGPase), starch synthase (SSS) and the transcription of Susy, AGP, SSS34 and SSS67 were enhanced by K application and had positive relationships with starch accumulation. Therefore, K application promoted starch accumulation and storage root yield through regulating the activities and genes transcription of SuSy, AGPase and SSS in the sucrose-to-starch conversion.

Highlights

It has been reported that K application of 240–300 kg K2 O ha−1 at basal was given high storage root yields [19], in accordance with the yield of sweet potato storage root (SPSR) observed in this study
It was found that the K application of 225–300 kg K2 O ha−1 could enhance the storage root yield by 28–30% and 46–48%
This study further analyzed the physiological mechanisms of K application to improve sweet potato yield

Summary

Introduction

Sweet potato (Ipomoea batatas (L.) Lam.), as a typical storage root crop, is the seventh most essential food crop in world, and is mainly used to produce starch and alcohol and to feed animals [1]. The sweet potato storage root (SPSR) yield depends on the number and the fresh weight of SPSR per plant, and is closely related to root growth and development and SPSR differentiation [2]. The differentiation of SPSR begins at 10–20 days after transplanting (DAT). The adventitious roots grew out of the stem base, and differentiate and enlarge into enlarged roots, and the number of SPSR per plant remains basically stable around 35 DAT [3]. For the fresh weight of SPSR per plant, starch is the major form of fixed carbon in SPSR, approximately 50–80% of SPSR’s dry biomass [4,5]

Methods

Results

Conclusion