Abstract
Abstract The optimization of culture medium with statistical methods is widely used in filamentous fungi glycosyl hydrolase production. The implementation of such methodology in bioreactors is very expensive as it requires several pH-controlled systems operating in parallel in order to test a large number of culture media components. The objective of this study was to evaluate potassium biphthalate buffer for pH control, which allows the optimization studies to be performed in shake flasks.The results have shown that buffering the culture medium with 0.1 M potassium biphthalate allowed pH control, resulting in a decrease of the standard deviation of triplicates for pH and activities of glycosyl hydrolase measurements. The use of this buffer allowed shake flask culture media optimization of enzyme production by Trichoderma harzianum, increasing the cellulase activity by more than 2 times compared to standard unbuffered culture medium. The same buffer can be used for culture media optimization of other fungi, such as Penicillium echinulatum.
Highlights
Cellulosic ethanol has been gaining increasing attention as an environmentally friendly biofuel with potential to compete with gasoline
Sugarcane bagasse pretreated by different methods (BEX, BED and BH) or standard micronized commercial cellulose Celufloc (CEL) were used for P. echinulatum
The objective was to evaluate the influence of potassium biphthalate buffer on the FPase enzyme activity and culture broth pH variation over time
Summary
Cellulosic ethanol has been gaining increasing attention as an environmentally friendly biofuel with potential to compete with gasoline. Production costs are still prohibitive and the main costs are related to enzyme production and enzymatic hydrolysis of lignocellulosic material The latter relies on the use of a plethora of glycosyl hydrolase enzyme complexes, which are mainly composed of cellobiohydrolase, endoglucanases and β-glucosidases in addition to a diversity of hemicellulases. Trichoderma, Penicillium, Aspergillus, among others, have been used industrially and academically for glycosyl hydrolase enzyme complex production (Delabona et al., 2013b; dos Reis et al, 2013; Pereira et al, 2013; Dillon et al, 2006) Filamentous fungi such as Trichoderma harzianum and Penicillium echinulatum have emerged as potential candidates for increasing the efficiency of glycosyl hydrolase enzymes complex production.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
