Abstract
BackgroundAtorvastatin (ATO) is an HMG‐CoA reductase inhibitor used to lower blood cholesterol, but it causes renal injury in high doses, including the kidney. The active ingredient of Nigella sativa, Thymoquinone (TQ), has antioxidant, antiapoptotic, and antiinflammatory properties.Aim of studyThe aim was to investigate whether posttreatment TQ could reverse ATO‐induced renal injury, and the possible mechanism of action by which TQ produces such effect.MethodsForty adult male Sprague Dawley rats were divided into 4 groups: control, TQ‐treated, ATO‐treated, and combined ATO/TQ‐treated. Rats were euthanized by cervical dislocation. Blood samples and kidneys were tested for kidney functions, oxidative stress, and apoptosis markers and for histopathological examination. Data was expressed as mean ± standard deviation (SD) and categorical variables are presented as percentage. One‐way analysis of variance (ANOVA) and post hoc tests (Bonferroni test) were used for the comparison of mean values between groups. P values of less than 0.01 were assumed to be significant (P<0.01). Data analysis was done using Study data were analyzed using SPSS.ResultsThe ATO‐treated group showed an increase in kidney functions, decreases in the oxidative stress markers, reduced glutathione and catalase, and increases in the protein carbonylation, lipid peroxidation, and caspase 3 activity. The ATO/TQ‐treated group appeared to improve these pathological changes. Morphometric analyses of the histopathological and ultrastructural examination supported these results.ConclusionPosttreatment TQ seemed to ameliorate oxidative stress‐induced renal injury produced by high‐dose ATO, suggesting a potential clinical application to the administration of high‐dose ATO, in patients with renal insufficiency.Support or Funding InformationSelf‐FundedThis abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.
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