Postnatal neurogenesis in the telencephalon of turtles: evidence for nonradial migration of new neurons from distant proliferative ventricular zones to the olfactory bulbs

Abstract

Postnatal neurogenesis in the turtle telencephalon was investigated by using bromodeoxyuridine immunocytochemistry and [ 3H]thymidine autoradiography. Red-eared slider turtles Trachemys scripta elegans (Cryptodira, Emydidae) 2–3 months old were injected with the thymidine analogue 5′-bromodeoxyuridine (BrdU) and allowed to survive for 7, 30, 90, and 180 days. Results indicate that cells in the walls of the lateral ventricles continue to proliferate postnatally. Shortly after BrdU treatment (seven days) most labelled cells were found in the walls of the lateral ventricles (ventricular zone: VZ). Labelled cells were particularly abundant in and around the ventricular sulci. The same pattern of labelling was found in the telencephalon of juvenile turtles (>two years old) injected with BrdU and killed seven days later, suggesting that the proliferative activity continues in the telencephalic VZ of turtles during juvenile stages of life and possibly into adulthood. With longer survival periods after BrdU administration (30, 90, and 180 days), the VZ of the telencephalon showed a similar pattern of labelling to that found at seven days. Furthermore, with survival periods of 90 and 180 days labelled cells resembling neurons were found in most telencephalic regions. The largest numbers of these putative neurons were found in the olfactory bulbs. By using [ 3H]thymidine autoradiography combined with electron microscopy these postnatally generated cells were confirmed as neurons. We conclude that postnatal neurogenesis occurs in the turtle telencephalon. This process is most prominent in the olfactory bulbs. From the pattern of proliferation of neuronal precursors in the VZ we infer that neurons recruited postnatally into the olfactory bulbs come from distant proliferative VZs in the walls of the lateral ventricles.