Postnatal maturation of the blood-brain barrier for unbound bilirubin in newborn piglets

Abstract

The postnatal maturation of the blood-brain barrier (BBB) for unbound bilirubin was studied in 2-day- and 2-week-old piglets. Hyperbilirubinemia was induced by bolus infusion of bilirubin at 25 mg/kg followed by continuous infusion of 20 mg/kg/h for 3 h. During the study period, arterial blood pH and blood gas tensions, serum osmolarity, and mean arterial blood pressures were within the physiologic range. Brain bilirubin content and the brain/blood distribution ratio for bilirubin were higher in the 2-day-old than in the 2-week-old piglets. In both age groups, regional brain bilirubin concentration and brain/blood ratios were higher in subcortical regions (cerebellum and brainstem) than in the cerebral cortex. We conclude that in the newborn piglets the blood-brain barrier for unbound bilirubin matures with increasing postnatal age and that irrespective of maturity the relative permeability of the BBB for bilirubin appears higher in subcortical than in cortical regions.