Abstract
Exploring the development of the hearing organ helps in the understanding of hearing and hearing impairments and it promotes the development of the regenerative approaches-based therapeutic efforts. The role of supporting cells in the development of the organ of Corti is much less elucidated than that of the cochlear sensory receptor cells. The use of our recently published method of single-cell electroporation loading of a fluorescent Ca2+ probe in the mouse hemicochlea preparation provided an appropriate means to investigate the Deiters’ cells at the subcellular level in two different cochlear turns (apical, middle). Deiters’ cell’s soma and process elongated, and the process became slimmer by maturation without tonotopic preference. The tonotopically heterogeneous spontaneous Ca2+ activity less frequently occurred by maturation and implied subcellular difference. The exogenous ATP- and UTP-evoked Ca2+ responses were maturation-dependent and showed P2Y receptor dominance in the apical turn. By monitoring the basic structural dimensions of this supporting cell type as well as its spontaneous and evoked purinergic Ca2+ signaling in the hemicochlea preparation in different stages in the critical postnatal P5-25 developmental period for the first time, we showed that the soma and the phalangeal process of the Deiters’ cells go through age- and tonotopy-dependent changes in the morphometric parameters and purinergic signaling.
Highlights
IntroductionExploring the development of the hearing organ helps to better understand the (patho)physiology of hearing and might contribute to develop novel regenerative therapeutic approaches [1,2] for restoring impaired hearing function [3]
Exploring the development of the hearing organ helps to better understand thephysiology of hearing and might contribute to develop novel regenerative therapeutic approaches [1,2] for restoring impaired hearing function [3].Mouse, the primary animal model in hearing research, to other altricial species, are deaf at birth [4,5]
Up to postnatal day 5–7 (P5–7), among others, important maturation steps are finalized in the inner (IHC) and outer hair cells (OHC) and their innervation [6,7,8,9]
Summary
Exploring the development of the hearing organ helps to better understand the (patho)physiology of hearing and might contribute to develop novel regenerative therapeutic approaches [1,2] for restoring impaired hearing function [3]. The primary animal model in hearing research, to other altricial species, are deaf at birth [4,5]. Up to postnatal day 5–7 (P5–7), among others, important maturation steps are finalized in the inner (IHC) and outer hair cells (OHC) and their innervation [6,7,8,9]. The first behavioural and auditory nerve responses that coincide with the opening of the outer ear can be observed at P10. In the 4–5 days, the hearing rapidly matures.
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