Postnatal development of the interstitial tissue of the rat kidney

Abstract

To study the ontogenetic development of the interstitial tissue of the kidney, rats were investigated 1, 3, 7, 14, 21 and 28 days after birth. Kidneys perfusion-fixed with glutaraldehyde were studied with light- and electron microscopy. Cryostate sections from kidneys immediately frozen in liquid nitrogen were studied with respect to the expression of MHC class II antigen using the monoclonal antibody OX6. The interstitial space of both the renal cortex and the outer and inner medulla was prominent during the first days postnatally. The relative interstitial volume of the cortex and outer part of the medulla then decreased in conjunction with the outgrowth and maturation of the superficial nephrons while the inner medullary interstitium remained wide. During the first postnatal days, the abundant interstitial cells of the cortex were connected via cytoplasmic processes to form a loose network which later became less well defined. The lipid-laden interstitial cells of the inner medulla showed essentially the same ultrastructure in the newborn as in the adult animal. Strong expression of class II antigen first appeared on epithelial cells of the thick ascending limb of Henle's loop about 7 days postnatally, and became weak at 28 days. From 21 days, a weak staining of the proximal tubules was also observed. While interstitial cells in the inner medulla were always negative, cortical and outer medullary interstitial cells became strongly positive for class II antigen from day 21 post partum.