Postnatal development of calbindin-D28k immunoreactivity in the cerebral cortex of the cat

Abstract

To learn about maturational patterns of nonpyramidal neurons in the cerebral cortex, calbindin-D28k immunoreactivity was studied in the kitten cortex. Immunoreactive neurons first appear in the cortical and subcortical areas related to the limbic system, including the cingulate and retrosplenial cortices, and in the secondary motor areas. These are followed by the primary motor and sensory association areas and, finally, by the primary sensory areas. In all cortical areas, calbindin-D28k immunoreactivity first develops in layer V pyramidal neurons and later in nonpyramidal neurons, except in the primary sensory areas, where immunoreactive pyramidal neurons are not found at any age. Transient calbindin-D28k immunoreactivity occurs in pyramidal neurons that are mainly localized in the cingulate and retrosplenial cortices and in the secondary motor area, as well as in nonpyramidal neurons localized in the subplate and layer I, and in a subset of large multipolar and bitufted neurons in layer VI. Nonpyramidal neurons localized in layers II to IV, and some neurons in layer VI, develop permanent calbindin-D28k immunoreactivity. Calbindin-D28k immunoreactivity labels subsets of GABAergic interneurons that form vertical axonal tufts, so that temporal and regional patterns of calbindin-D28k immunoreactivity during development may be implicated in the maturation of columnar (vertical) inhibition in the cerebral cortex. In addition to neurons, corticofugal and afferent fibres of subcortical origin exhibit calbindin-D28k immunoreactivity. Transient calbindin-D28k immunoreactivity occurs in corticofugal fibres arising from the cingulate and prefrontal cortices, which are probably corticostriatal projection fibres. In contrast, permanent immunoreactivity occurs in what are probably thalamocortical fibres ending in layer IV, and in punctate terminals located in the upper third of layer I.