Postnatal Changes in Mitochondrial Protein Mass and Respiration in Skeletal Muscle from the Newborn Pig

Abstract

Quantitative and functional changes occurring in mitochondria were studied in pig skeletal muscle between birth and 5 days of life. Postnatal changes were followed separately on intermyofibrillar and subsarcolemmal mitochondria isolated from rhomboı̈deus (RH) and longissimus dorsi (LD) muscles. The integrity and purity of the isolated mitochondria was checked by electron microscopic observations. The mass of mitochondrial protein was not different between muscles at birth. It increased tremendously during the first 5 days of life, by 49% in LD (P < 0.001) and 93% in RH (P < 0.001) muscle and was 30% higher in RH than in LD muscle at 5 days of life (P < 0.05). Mitochondria isolated from RH muscle exhibited 30% higher oxidative and phosphorylative capacities than those from LD muscle at 5 days of life (P < 0.05). Intermyofibrillar (IM) mitochondria had high respiration rate, enzyme activities and coupling parameters (respiratory control ratio, phosphorus-oxygen ratio) from birth. Subsarcolemmal (SS) mitochondria were less active than IM mitochondria; their respiration rate and enzyme activities were 60% lower (P < 0.01) and increased with age, particularly in LD muscle (P < 0.05). Short-term cold exposure had no effect on mitochondrial mass and activity. These results suggest that muscle mitochondria are functional from birth and are changing primarily quantitatively. SS and IM mitochondria exhibit specific changes that are probably involved in the postnatal acquisition of skeletal muscle oxidative metabolism.