Poster 43: Sodium Iodoacetate Induced Osteoarthritis Model in Rabbit Temporomandibular Joint

Abstract

Statement of the Problem: A series of experimental studies showed that surgical induction of anterior disc displacement led to the degenerative changes in the condylar cartilage. However, this surgical procedure induced not only mechanical alteration but also artificial surgical damage to the joint structures. Structural models generate osteoarthritic changes similar to those observed in humans by intra-articular injection of drugs targeting articular cells, mainly condrocytes. Chondrocyte metabolism inhibitor, MIA, has been reported to induce the disruption of glycolysis and subsequently cell death and the loss of chondrocytes. MIA injection induced to various histological changes in the knee joints closely resemble to human osteoarthritis (OA). Although the biological action of this drug has been well documented in the knee joints, only one animal study reported the injection of 1.5 mg MIA into the joint without disc derangement and joint capsule opening. The aim of this study was to determine MIA induced osteoarthritis in rabbit TMJ for using different dosage and time intervals. The null hypothesis is to find the most appropriate dosage of MIA and shortest time to induce osteoarthritis in TMJ without surgery and joint capsule opening like as clinically usage of arthrocentesis in humans. For this purpose, we investigated radiologic, histologic and histomorphometric findings of the four different concentrations of MIA (1.5, 2, 2.5, 3 mg/mL) to create osteoarthritic changes in rabbit TMJ within 3 different time intervals (2, 4 and 6 weeks).