Abstract
Objective: To explore whether the transplanted human adipose tissue–derived stem cells (hATSCs) could survive and improve functional recovery after contusive spinal cord injury (SCI) in rats. Design: Case-controlled study. Setting: University laboratory. Animals: 30 female Sprague-Dawley rats (weight range, 250–350g). Interventions: hATSCs, which were isolated from male human liposuctioned tissues, were injected with 20μL of growth medium into 15 female rats and only 20μL of growth medium were injected into the remaining 15 rats used as controls. The procedure was performed 1 week after making contusive SCI by weight drop device. Main Outcome Measures: The functional outcome was evaluated by using the Basso-Beattie-Bresnahan motor rating score and inclined plane test. The histologic morphology was observed by optical microscope with cross-sectional area of the spinal cord. The distribution of donor cells was measured by identifying Y chromosome-positive cells using fluorescent in situ hybridization. Results: hATSCs significantly improved motor recovery compared with the control group 4 weeks after transplantation. The morphometric changes were also better in the hATSCs group. The transplanted cells were partially engrafted into the parenchyma of the injured spinal cord. Conclusions: These data suggest that transplanted hATSCs may have a therapeutic role after SCI.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
